Label-Free Multimetric Measurement of Molecular Binding Kinetics by Electrical Modulation of a Flexible Nanobiolayer

Xiaoyan Zhou, Guangzhong Ma, Zijian Wan, Shaopeng Wang

Research output: Contribution to journalArticlepeer-review

Abstract

Most label-free techniques rely on measuring refractive index or mass change on the sensor surface. Thus, it is challenging for them to measure small molecules or enzymatic processes that only induce a minor mass change on the analyte molecules. Here, we have developed a technique by combining Surface Plasmon Resonance sensing with an Oscillating Biomolecule Layer approach (SPR-OBL) to enhance the sensitivity of traditional SPR. In addition to the inherent mass sensitivity, SPR-OBL is also sensitive to the charge and conformational change of the analyte; hence it overcomes the mass limit and is able to detect small molecules. We show that the multimetric SPR-OBL measurement allows for sensing any changes regarding mass, charge, and conformation, which expands the detection capability of SPR.

Original languageEnglish (US)
Pages (from-to)3461-3469
Number of pages9
JournalACS sensors
Volume7
Issue number11
DOIs
StatePublished - Nov 25 2022
Externally publishedYes

Keywords

  • charge sensitive
  • drug screening
  • nanodisc
  • phosphorylation
  • small molecule detection
  • Surface plasmon resonance

ASJC Scopus subject areas

  • Bioengineering
  • Instrumentation
  • Process Chemistry and Technology
  • Fluid Flow and Transfer Processes

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