Abstract

Membrane proteins mediate a variety of cellular responses to extracellular signals. Although membrane proteins are studied intensively for their values as disease biomarkers and therapeutic targets, in situ investigation of the binding kinetics of membrane proteins with their ligands has been a challenge. Traditional approaches isolate membrane proteins and then study them ex situ, which does not reflect accurately their native structures and functions. We present a label-free plasmonic microscopy method to map the local binding kinetics of membrane proteins in their native environment. This analytical method can perform simultaneous plasmonic and fluorescence imaging, and thus make it possible to combine the strengths of both label-based and label-free techniques in one system. Using this method, we determined the distribution of membrane proteins on the surface of single cells and the local binding kinetic constants of different membrane proteins. Furthermore, we studied the polarization of the membrane proteins on the cell surface during chemotaxis.

Original languageEnglish (US)
Pages (from-to)846-853
Number of pages8
JournalNature Chemistry
Volume4
Issue number10
DOIs
StatePublished - Oct 2012

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Labels
Membrane Proteins
Cells
Proteins
Membranes
Kinetics
Biomarkers
Microscopic examination
Fluorescence
Ligands
Polarization
Imaging techniques

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Label-free measuring and mapping of binding kinetics of membrane proteins in single living cells. / Wang, Wei; Yang, Yunze; Wang, Shaopeng; Nagaraj, Vinay J.; Liu, Qiang; Wu, Jie; Tao, Nongjian.

In: Nature Chemistry, Vol. 4, No. 10, 10.2012, p. 846-853.

Research output: Contribution to journalArticle

Wang, Wei ; Yang, Yunze ; Wang, Shaopeng ; Nagaraj, Vinay J. ; Liu, Qiang ; Wu, Jie ; Tao, Nongjian. / Label-free measuring and mapping of binding kinetics of membrane proteins in single living cells. In: Nature Chemistry. 2012 ; Vol. 4, No. 10. pp. 846-853.
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AU - Wu, Jie

AU - Tao, Nongjian

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