Label free detection of NAPPA via mass spectrometry

Rosanna Spera, Francesco Badino, Eugenie Hainsworth, Manuel Fuentes, Sanjeeva Srivastava, Joshua LaBaer, Claudio Nicolini

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Citations (Scopus)

Abstract

Our research relates to the implementation of Nucleic Acid Programmable Protein Array (NAPPA) analysis by matrix assisted laser desorption/ionization mass spectrometry (MALDI MS). Such analysis enables interrogation of protein-protein interactions in a label- free manner by desorption and ionization of analytes (e.g., proteins synthesized from the immobilized cDNA and/or protein bounded to these). Mass spectrometry, in fact, has the unique advantage of being able to determine not only the presence but also establish the identity of a given ligand. However, the development of a MALDI MS-compatible protein microarray is complex since existing methods for forming protein microarrays do not transfer readily onto a MALDI target. Here we present a novel method for implementing a procedure to analyze protein contents on NAPPA protein microarrays using MALDI TOF mass spectrometry.

Original languageEnglish (US)
Title of host publicationFunctional Proteomics and Nanotechnology-Based Microarrays
PublisherPan Stanford Publishing Pte. Ltd.
Pages61-78
Number of pages18
ISBN (Print)9789814267762
DOIs
StatePublished - Jun 1 2010
Externally publishedYes

Fingerprint

Protein Array Analysis
Nucleic Acids
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Mass spectrometry
Labels
Mass Spectrometry
Proteins
Microarrays
Ionization
Desorption
Immobilized Proteins
Complementary DNA
Ligands
Lasers
Research

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Spera, R., Badino, F., Hainsworth, E., Fuentes, M., Srivastava, S., LaBaer, J., & Nicolini, C. (2010). Label free detection of NAPPA via mass spectrometry. In Functional Proteomics and Nanotechnology-Based Microarrays (pp. 61-78). Pan Stanford Publishing Pte. Ltd.. https://doi.org/10.4032/9789814267779

Label free detection of NAPPA via mass spectrometry. / Spera, Rosanna; Badino, Francesco; Hainsworth, Eugenie; Fuentes, Manuel; Srivastava, Sanjeeva; LaBaer, Joshua; Nicolini, Claudio.

Functional Proteomics and Nanotechnology-Based Microarrays. Pan Stanford Publishing Pte. Ltd., 2010. p. 61-78.

Research output: Chapter in Book/Report/Conference proceedingChapter

Spera, R, Badino, F, Hainsworth, E, Fuentes, M, Srivastava, S, LaBaer, J & Nicolini, C 2010, Label free detection of NAPPA via mass spectrometry. in Functional Proteomics and Nanotechnology-Based Microarrays. Pan Stanford Publishing Pte. Ltd., pp. 61-78. https://doi.org/10.4032/9789814267779
Spera R, Badino F, Hainsworth E, Fuentes M, Srivastava S, LaBaer J et al. Label free detection of NAPPA via mass spectrometry. In Functional Proteomics and Nanotechnology-Based Microarrays. Pan Stanford Publishing Pte. Ltd. 2010. p. 61-78 https://doi.org/10.4032/9789814267779
Spera, Rosanna ; Badino, Francesco ; Hainsworth, Eugenie ; Fuentes, Manuel ; Srivastava, Sanjeeva ; LaBaer, Joshua ; Nicolini, Claudio. / Label free detection of NAPPA via mass spectrometry. Functional Proteomics and Nanotechnology-Based Microarrays. Pan Stanford Publishing Pte. Ltd., 2010. pp. 61-78
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