Label free detection of NAPPA via mass spectrometry

Rosanna Spera, Francesco Badino, Eugenie Hainsworth, Manuel Fuentes, Sanjeeva Srivastava, Joshua LaBaer, Claudio Nicolini

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Scopus citations

Abstract

Our research relates to the implementation of Nucleic Acid Programmable Protein Array (NAPPA) analysis by matrix assisted laser desorption/ionization mass spectrometry (MALDI MS). Such analysis enables interrogation of protein-protein interactions in a label- free manner by desorption and ionization of analytes (e.g., proteins synthesized from the immobilized cDNA and/or protein bounded to these). Mass spectrometry, in fact, has the unique advantage of being able to determine not only the presence but also establish the identity of a given ligand. However, the development of a MALDI MS-compatible protein microarray is complex since existing methods for forming protein microarrays do not transfer readily onto a MALDI target. Here we present a novel method for implementing a procedure to analyze protein contents on NAPPA protein microarrays using MALDI TOF mass spectrometry.

Original languageEnglish (US)
Title of host publicationFunctional Proteomics and Nanotechnology-Based Microarrays
PublisherPan Stanford Publishing Pte. Ltd.
Pages61-78
Number of pages18
ISBN (Print)9789814267762
DOIs
StatePublished - Jun 1 2010
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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