Abstract

An immunosensor for rapid and low level detection of the bacterial pathogen Salmonella enterica Serovar Typhimurium was designed and developed based upon label-free electrochemical impedance spectroscopy and correlated to viable cell counts. The immunosensor was fabricated by electroplating gold onto a disposable printed circuit board (PCB) electrode by immobilizing monoclonal antibody (MAb) specific against Salmonella typhimurium cell surface lipopolysaccharide (LPS) onto the surface of the electrode. Use of mass-fabricated and electroplated PCB electrodes allowed for disposable, highly sensitive, and rapid detection of Salmonella in an aqueous environment. Results demonstrate that in purified solution, Salmonella can be detected as low as 10 CFU in a 100 mL volume and label-free and rapid manner in fewer than 90 s. The cost effective approach described here can be used for detection of pathogens with relevance for healthcare, food, and environmental applications.

Original languageEnglish (US)
Pages (from-to)2267-2271
Number of pages5
JournalElectroanalysis
Volume21
Issue number20
DOIs
StatePublished - Oct 2009

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Salmonella
Electrochemical impedance spectroscopy
Labels
Immunosensors
Pathogens
Printed circuit boards
Electrodes
Monoclonal antibodies
Electroplating
Gold
Lipopolysaccharides
Monoclonal Antibodies
Costs

Keywords

  • Antibodies
  • Impedance spectroscopy
  • Label-free detection
  • Medicinal chemistry
  • Printed-circuit-board
  • Salmonella

ASJC Scopus subject areas

  • Analytical Chemistry
  • Electrochemistry

Cite this

Label-free and ultra-low level detection of salmonella enterica serovar typhimurium using electrochemical impedance spectroscopy. / LaBelle, Jeffrey; Shah, Miti; Reed, Justin; Nandakumar, Vivek; Alford, Terry; Wilson, James W.; Nickerson, Cheryl; Joshi, Lokesh.

In: Electroanalysis, Vol. 21, No. 20, 10.2009, p. 2267-2271.

Research output: Contribution to journalArticle

LaBelle, Jeffrey ; Shah, Miti ; Reed, Justin ; Nandakumar, Vivek ; Alford, Terry ; Wilson, James W. ; Nickerson, Cheryl ; Joshi, Lokesh. / Label-free and ultra-low level detection of salmonella enterica serovar typhimurium using electrochemical impedance spectroscopy. In: Electroanalysis. 2009 ; Vol. 21, No. 20. pp. 2267-2271.
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