Kinetics of phyllosemiquinone oxidation in the Photosystem i reaction centre of Acaryochloris marina

Stefano Santabarbara, Benjamin Bailleul, Kevin Redding, James Barber, Fabrice Rappaport, Alison Telfer

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    Light-induced electron transfer reactions in the chlorophyll a/d-binding Photosystem I reaction centre of Acaryochloris marina were investigated in whole cells by pump-probe optical spectroscopy with a temporal resolution of ~ 5 ns at room temperature. It is shown that phyllosemiquinone, the secondary electron transfer acceptor anion, is oxidised with bi-phasic kinetics characterised by lifetimes of 88 ± 6 ns and 345 ± 10 ns. These lifetimes, particularly the former, are significantly slower than those reported for chlorophyll a-binding Photosystem I, which typically range in the 5-30 ns and 200-300 ns intervals. The possible mechanism of electron transfer reactions in the chlorophyll a/d-binding Photosystem I and the slower oxidation kinetics of the secondary acceptors are discussed.

    Original languageEnglish (US)
    Pages (from-to)328-335
    Number of pages8
    JournalBiochimica et Biophysica Acta - Bioenergetics
    Volume1817
    Issue number2
    DOIs
    StatePublished - Feb 2012

    Fingerprint

    Marinas
    Photosystem I Protein Complex
    Electrons
    Oxidation
    Kinetics
    Anions
    Spectrum Analysis
    Pumps
    Light
    Temperature
    chlorophyll a
    chlorophyll d

    Keywords

    • Acaryochloris marina
    • Bidirectional electron transfer
    • Chlorophyll d
    • Photosystem I
    • Phylloquinone
    • Radical pair

    ASJC Scopus subject areas

    • Biochemistry
    • Biophysics
    • Cell Biology

    Cite this

    Kinetics of phyllosemiquinone oxidation in the Photosystem i reaction centre of Acaryochloris marina. / Santabarbara, Stefano; Bailleul, Benjamin; Redding, Kevin; Barber, James; Rappaport, Fabrice; Telfer, Alison.

    In: Biochimica et Biophysica Acta - Bioenergetics, Vol. 1817, No. 2, 02.2012, p. 328-335.

    Research output: Contribution to journalArticle

    Santabarbara, Stefano ; Bailleul, Benjamin ; Redding, Kevin ; Barber, James ; Rappaport, Fabrice ; Telfer, Alison. / Kinetics of phyllosemiquinone oxidation in the Photosystem i reaction centre of Acaryochloris marina. In: Biochimica et Biophysica Acta - Bioenergetics. 2012 ; Vol. 1817, No. 2. pp. 328-335.
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    AU - Barber, James

    AU - Rappaport, Fabrice

    AU - Telfer, Alison

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