Kinetics of phyllosemiquinone oxidation in the Photosystem i reaction centre of Acaryochloris marina

Stefano Santabarbara, Benjamin Bailleul, Kevin Redding, James Barber, Fabrice Rappaport, Alison Telfer

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Light-induced electron transfer reactions in the chlorophyll a/d-binding Photosystem I reaction centre of Acaryochloris marina were investigated in whole cells by pump-probe optical spectroscopy with a temporal resolution of ~ 5 ns at room temperature. It is shown that phyllosemiquinone, the secondary electron transfer acceptor anion, is oxidised with bi-phasic kinetics characterised by lifetimes of 88 ± 6 ns and 345 ± 10 ns. These lifetimes, particularly the former, are significantly slower than those reported for chlorophyll a-binding Photosystem I, which typically range in the 5-30 ns and 200-300 ns intervals. The possible mechanism of electron transfer reactions in the chlorophyll a/d-binding Photosystem I and the slower oxidation kinetics of the secondary acceptors are discussed.

Original languageEnglish (US)
Pages (from-to)328-335
Number of pages8
JournalBiochimica et Biophysica Acta - Bioenergetics
Volume1817
Issue number2
DOIs
StatePublished - Feb 2012

Fingerprint

Marinas
Photosystem I Protein Complex
Electrons
Oxidation
Kinetics
Anions
Spectrum Analysis
Pumps
Light
Temperature
chlorophyll a
chlorophyll d

Keywords

  • Acaryochloris marina
  • Bidirectional electron transfer
  • Chlorophyll d
  • Photosystem I
  • Phylloquinone
  • Radical pair

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology

Cite this

Kinetics of phyllosemiquinone oxidation in the Photosystem i reaction centre of Acaryochloris marina. / Santabarbara, Stefano; Bailleul, Benjamin; Redding, Kevin; Barber, James; Rappaport, Fabrice; Telfer, Alison.

In: Biochimica et Biophysica Acta - Bioenergetics, Vol. 1817, No. 2, 02.2012, p. 328-335.

Research output: Contribution to journalArticle

Santabarbara, Stefano ; Bailleul, Benjamin ; Redding, Kevin ; Barber, James ; Rappaport, Fabrice ; Telfer, Alison. / Kinetics of phyllosemiquinone oxidation in the Photosystem i reaction centre of Acaryochloris marina. In: Biochimica et Biophysica Acta - Bioenergetics. 2012 ; Vol. 1817, No. 2. pp. 328-335.
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