Kinetics of a hydrogen-oxidizing, perchlorate-reducing bacterium

Robert Nerenberg, Yasunori Kawagoshi, Bruce Rittmann

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

This paper provides the first kinetic parameters for a hydrogen-oxidizing perchlorate-reducing bacterium (PCRB), Dechloromonas sp. PC1. The qmax for perchlorate and chlorate were 3.1 and 6.3 mg/mgDW-day, respectively. The K for perchlorate was 0.14 mg/L, an order of magnitude lower than reported for other PCRB. The yields Y on perchlorate and chlorate were 0.23 and 0.22 mgDW/mg, respectively, and the decay constant b was 0.055/day. The growth-threshold, Smin, for perchlorate was 14 μg/L, suggesting that perchlorate cannot be reduced below this level when perchlorate is the primary electron-acceptor, although it may be possible when oxygen or nitrate is the primary acceptor. Chlorate accumulated at maximum concentrations of 0.6-4.3 mg/L in batch tests with initial perchlorate concentrations ranging from 100 to 600 mg/L. Furthermore, 50 mg/L chlorate inhibited perchlorate reduction with perchlorate at 100 mg/L. This is the first report of chlorate accumulation and inhibition for a pure culture of PCRB. These Chlorate effects are consistent with competitive inhibition between perchlorate and chlorate for the (per)chlorate reductase enzyme.

Original languageEnglish (US)
Pages (from-to)3290-3296
Number of pages7
JournalWater Research
Volume40
Issue number17
DOIs
StatePublished - Oct 2006

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perchlorate
Bacteria
hydrogen
kinetics
Hydrogen
bacterium
Kinetics
Kinetic parameters
Nitrates
Enzymes
Oxygen
Electrons

Keywords

  • Biodegradation
  • Chlorate
  • Competitive inhibition
  • Hydrogen
  • Kinetics
  • Perchlorate
  • S

ASJC Scopus subject areas

  • Earth-Surface Processes

Cite this

Kinetics of a hydrogen-oxidizing, perchlorate-reducing bacterium. / Nerenberg, Robert; Kawagoshi, Yasunori; Rittmann, Bruce.

In: Water Research, Vol. 40, No. 17, 10.2006, p. 3290-3296.

Research output: Contribution to journalArticle

Nerenberg, Robert ; Kawagoshi, Yasunori ; Rittmann, Bruce. / Kinetics of a hydrogen-oxidizing, perchlorate-reducing bacterium. In: Water Research. 2006 ; Vol. 40, No. 17. pp. 3290-3296.
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