Kinetic characterization of Methanobacterium bryantii M.o.H.

Fatih Karadagli, Bruce Rittmann

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

H2 is a key electron donor for many anaerobic microorganisms; thus, keen competition for H2 occurs among H2-utilizing microbial groups. Monod kinetic parameters provide essential information for kinetic analysis of competition for H2. In this study, we estimated Monod kinetic parameter values for a methanogen that consumes only H2 as its electron donor, Methanobacterium bryantii M.o.H. Utilization of a single electron donor is an advantage in this study, because complications from alternate metabolic pathways are avoided. Using a set of batch experiments designed to provide the best estimates of each parameter, we obtained these values: maximum specific growth rate (μmax) = 0.77/day, maximum substrate consumption rate (qmax) = 2.36 mol-H2/gcells/ day, true yield (Y) = 0.325 gcell/mol H2, fraction of donor electrons to synthesis (fs°) = 0.03 e-cell/e-donor, half-maximum-rate substrate concentration (Ks) = 18 000 nM = 18 μM H2, and endogenous decay rate (b) = 0.088/day. This self-consistent set of parameters indicates that, when H2 is not limiting, M. bryantii M.o.H. is a slow grower (low μmax) compared to other H2-oxidizing methanogens and sulfate reducers, and this is mainly due to its low true Y, not a low qmax. The relatively high Ks and b values suggest that M. bryantii also may not be a strong competitor when H2 is limiting.

Original languageEnglish (US)
Pages (from-to)4900-4905
Number of pages6
JournalEnvironmental Science and Technology
Volume39
Issue number13
DOIs
StatePublished - Jul 1 2005

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Methanogens
electron
kinetics
Kinetics
Electrons
Kinetic parameters
substrate
Substrates
Microorganisms
Sulfates
microorganism
sulfate
parameter
rate
experiment
Experiments
Metabolic Networks and Pathways
analysis
consumption

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Science(all)
  • Environmental Chemistry

Cite this

Kinetic characterization of Methanobacterium bryantii M.o.H. / Karadagli, Fatih; Rittmann, Bruce.

In: Environmental Science and Technology, Vol. 39, No. 13, 01.07.2005, p. 4900-4905.

Research output: Contribution to journalArticle

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