Kinetic behaviour of dopamine-polyphenol oxidase on electrodes of tetrathiafulvalenium tetracyanoquinodimethanide and tetracyanoquinodimethane species

E. S. Forzani, G. A. Rivas, V. M. Solis

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

This paper presents a study of the electrochemical behaviour of polyphenol oxidase onto conducting organic salt electrodes using dopamine as enzymatic substrate. The enzyme is immobilized behind a dialysis membrane. TTF·TCNQ and carbon paste with TCNQ0 and NaTCNQ are employed, and the role of the different salt components as enzyme regeneration mediators is analysed under anaerobic conditions. It is concluded that both TCNQ0 and NaTCNQ species are able to regenerate the active enzyme although chemical reactions between the species make it difficult to discriminate their behaviour. Results strongly suggest a heterogeneous mechanism for the enzymatic reaction and NaTCNQ as enzymatic regenerator on TTF·TCNQ. Apparent Michaelis-Menten constants are evaluated. The results clearly point to an enzymatic control of the reaction rate under our experimental conditions. A chemical catalytic effect due to coupled chemical reactions is also described. The biosensors analysed are potentially useful from the analytical point of view. Different electrode materials were analysed: TTF·TCNQ and carbon paste with TCNQ0 and NaTCNQ.

Original languageEnglish (US)
Pages (from-to)174-183
Number of pages10
JournalJournal of Electroanalytical Chemistry
Volume461
Issue number1-2
DOIs
StatePublished - Jan 1 1999
Externally publishedYes

Keywords

  • Bioelectrodes
  • Dopamine
  • Organic conducting salts
  • Polyphenol oxidase
  • Tetrathiafulvalenium tetracyanoquinodimethanide

ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemical Engineering(all)
  • Electrochemistry

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