@article{9bac9deb07cd4514861ec7bdee67b916,
title = "Isotope labeling of mammalian GPCRs in HEK293 cells and characterization of the C-terminus of bovine rhodopsin by high resolution liquid NMR spectroscopy",
abstract = "High amino acid coverage labeling of the mammalian G protein coupled receptors (GPCR) rhodopsin was established with 15N and 15N/13C isotopes. Rhodopsin was expressed at preparative scale in HEK293S cells and studied in full-length by NMR spectroscopy in detergent micelle solution. This resulted in the assignment and detailed study of the dynamic properties of the C-terminus of rhodopsin. The rhodopsin C-terminus is immobilized until Ala333, after which it becomes unstructured.",
keywords = "Isotope labeling, Mammalian GPCR, NMR spectroscopy, Rhodopsin",
author = "Karla Werner and Christian Richter and Judith Klein-Seetharaman and Harald Schwalbe",
note = "Funding Information: Acknowledgments We thank for the financial support from the Sofya Kovalevskaya-Award granted by the Alexander von Humboldt-Foundation through the Investment in the Future Programme of the German Federal Government. The work was supported by DFG (SFB 628: {\textquoteleft}{\textquoteleft}Functional Membrane Proteomics{\textquoteright}{\textquoteright}), the State of Hesse (BMRZ) and the Fonds der Chemischen Industrie (H.S.). We acknowledge support by Cambridge Isotope Laboratories with isotope labeled material.",
year = "2008",
month = jan,
doi = "10.1007/s10858-007-9205-3",
language = "English (US)",
volume = "40",
pages = "49--53",
journal = "Journal of Biomolecular NMR",
issn = "0925-2738",
publisher = "Springer Netherlands",
number = "1",
}