Isotope labeling of mammalian GPCRs in HEK293 cells and characterization of the C-terminus of bovine rhodopsin by high resolution liquid NMR spectroscopy

Karla Werner, Christian Richter, Judith Klein-Seetharaman, Harald Schwalbe

Research output: Contribution to journalArticlepeer-review

52 Scopus citations

Abstract

High amino acid coverage labeling of the mammalian G protein coupled receptors (GPCR) rhodopsin was established with 15N and 15N/13C isotopes. Rhodopsin was expressed at preparative scale in HEK293S cells and studied in full-length by NMR spectroscopy in detergent micelle solution. This resulted in the assignment and detailed study of the dynamic properties of the C-terminus of rhodopsin. The rhodopsin C-terminus is immobilized until Ala333, after which it becomes unstructured.

Original languageEnglish (US)
Pages (from-to)49-53
Number of pages5
JournalJournal of Biomolecular NMR
Volume40
Issue number1
DOIs
StatePublished - Jan 2008
Externally publishedYes

Keywords

  • Isotope labeling
  • Mammalian GPCR
  • NMR spectroscopy
  • Rhodopsin

ASJC Scopus subject areas

  • Biochemistry
  • Spectroscopy

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