Isotope labeling in mammalian cells

Arpana Dutta, Krishna Saxena, Harald Schwalbe, Judith Klein-Seetharaman

Research output: Chapter in Book/Report/Conference proceedingChapter

27 Scopus citations

Abstract

Isotope labeling of proteins represents an important and often required tool for the application of nuclear magnetic resonance (NMR) spectroscopy to investigate the structure and dynamics of proteins. Mammalian expression systems have conventionally been considered to be too weak and inefficient for protein expression. However, recent advances have significantly improved the expression levels of these systems. Here, we provide an overview of some of the recent developments in expression strategies for mammalian expression systems in view of NMR investigations.

Original languageEnglish (US)
Title of host publicationProtein NMR Techniques
Pages55-69
Number of pages15
DOIs
StatePublished - 2012
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume831
ISSN (Print)1064-3745

Keywords

  • Human embryonic kidney cells
  • Isotope labeling
  • Nuclear magnetic resonance
  • Recombinant protein expression

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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