Isotope labeling in insect cells

Krishna Saxena, Arpana Dutta, Judith Klein-Seetharaman, Harald Schwalbe

Research output: Chapter in Book/Report/Conference proceedingChapter

25 Scopus citations

Abstract

Recent years have seen remarkable progress in applying nuclear magnetic resonance (NMR) spectroscopy to proteins that have traditionally been difficult to study due to issues with folding, posttranslational modification, and expression levels or combinations thereof. In particular, insect cells have proved useful in allowing large quantities of isotope-labeled, functional proteins to be obtained and purified to homogeneity, allowing study of their structures and dynamics by using NMR. Here, we provide protocols that have proven successful in such endeavors.

Original languageEnglish (US)
Title of host publicationProtein NMR Techniques
Pages37-54
Number of pages18
DOIs
StatePublished - 2012
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume831
ISSN (Print)1064-3745

Keywords

  • Baculovirus
  • Insect cells
  • Isotope labeling
  • Nuclear magnetic resonance
  • Recombinant protein expression

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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