Isolation of yeast artificial chromosome clones from 54 polymorphic loci mapped with high odds on human chromosome 4

Jlan Bing Fan; Joe Deyoung; Rob Lagacé; R. Andrea Lina; Zhldong Xu; Jeffrey C. Murray; Kenneth H. Buetow; Jean Weissenbach; Richard D. Goold; David R. Cox; Richard M. Myers

doi:10.1093/hmg/3.2.243

Isolation of yeast artificial chromosome clones from 54 polymorphic loci mapped with high odds on human chromosome 4

Jlan Bing Fan, Joe Deyoung, Rob Lagacé, R. Andrea Lina, Zhldong Xu, Jeffrey C. Murray, Kenneth H. Buetow, Jean Weissenbach, Richard D. Goold, David R. Cox, Richard M. Myers

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

We constructed a yeast artificial chromosome (YAC) framework map of human chromosome 4 by screening a YAC library with 63 polymorphic DNA markers located on the chromosome. These genetic markers are from two framework melotic maps that had previously been constructed by two research groups, and are placed on the two maps with odds for their order of 1000: 1 or greater. In addition to Isolating and determining the sizes of 141 YAC clones for 54 of these markers, we combined the two framework melotic maps to produce a single Integrated map. These combined maps and the YAC clones provide a set of extended DNA loci ordered at high odds that can be used to isolate additional polymorphic loci and genes, and to serve as a framework for obtaining a higher resolution physical map of the chromosome.

Original language	English (US)
Pages (from-to)	243-246
Number of pages	4
Journal	Human Molecular Genetics
Volume	3
Issue number	2
DOIs	https://doi.org/10.1093/hmg/3.2.243
State	Published - Feb 1994
Externally published	Yes

ASJC Scopus subject areas

Molecular Biology
Genetics
Genetics(clinical)

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title = "Isolation of yeast artificial chromosome clones from 54 polymorphic loci mapped with high odds on human chromosome 4",

abstract = "We constructed a yeast artificial chromosome (YAC) framework map of human chromosome 4 by screening a YAC library with 63 polymorphic DNA markers located on the chromosome. These genetic markers are from two framework melotic maps that had previously been constructed by two research groups, and are placed on the two maps with odds for their order of 1000: 1 or greater. In addition to Isolating and determining the sizes of 141 YAC clones for 54 of these markers, we combined the two framework melotic maps to produce a single Integrated map. These combined maps and the YAC clones provide a set of extended DNA loci ordered at high odds that can be used to isolate additional polymorphic loci and genes, and to serve as a framework for obtaining a higher resolution physical map of the chromosome.",

author = "Fan, {Jlan Bing} and Joe Deyoung and Rob Lagac{\'e} and Lina, {R. Andrea} and Zhldong Xu and Murray, {Jeffrey C.} and Buetow, {Kenneth H.} and Jean Weissenbach and Goold, {Richard D.} and Cox, {David R.} and Myers, {Richard M.}",

note = "Funding Information: We thank Denis Le Paslier and Daniel Cohen (CEPH, France) for providing the CEPH Mark I human genomic YAC library, Laura Stuve for comments on the manuscript, and our colleagues at the Human Genome Mapping Center at Stanford University for their contributions to this work. This work was supported by NIH Grant 5P50HG00206.",

year = "1994",

month = feb,

doi = "10.1093/hmg/3.2.243",

language = "English (US)",

volume = "3",

pages = "243--246",

journal = "Human Molecular Genetics",

issn = "0964-6906",

publisher = "Oxford University Press",

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T1 - Isolation of yeast artificial chromosome clones from 54 polymorphic loci mapped with high odds on human chromosome 4

AU - Fan, Jlan Bing

AU - Deyoung, Joe

AU - Lagacé, Rob

AU - Lina, R. Andrea

AU - Xu, Zhldong

AU - Murray, Jeffrey C.

AU - Buetow, Kenneth H.

AU - Weissenbach, Jean

AU - Goold, Richard D.

AU - Cox, David R.

AU - Myers, Richard M.

N1 - Funding Information: We thank Denis Le Paslier and Daniel Cohen (CEPH, France) for providing the CEPH Mark I human genomic YAC library, Laura Stuve for comments on the manuscript, and our colleagues at the Human Genome Mapping Center at Stanford University for their contributions to this work. This work was supported by NIH Grant 5P50HG00206.

PY - 1994/2

Y1 - 1994/2

N2 - We constructed a yeast artificial chromosome (YAC) framework map of human chromosome 4 by screening a YAC library with 63 polymorphic DNA markers located on the chromosome. These genetic markers are from two framework melotic maps that had previously been constructed by two research groups, and are placed on the two maps with odds for their order of 1000: 1 or greater. In addition to Isolating and determining the sizes of 141 YAC clones for 54 of these markers, we combined the two framework melotic maps to produce a single Integrated map. These combined maps and the YAC clones provide a set of extended DNA loci ordered at high odds that can be used to isolate additional polymorphic loci and genes, and to serve as a framework for obtaining a higher resolution physical map of the chromosome.

AB - We constructed a yeast artificial chromosome (YAC) framework map of human chromosome 4 by screening a YAC library with 63 polymorphic DNA markers located on the chromosome. These genetic markers are from two framework melotic maps that had previously been constructed by two research groups, and are placed on the two maps with odds for their order of 1000: 1 or greater. In addition to Isolating and determining the sizes of 141 YAC clones for 54 of these markers, we combined the two framework melotic maps to produce a single Integrated map. These combined maps and the YAC clones provide a set of extended DNA loci ordered at high odds that can be used to isolate additional polymorphic loci and genes, and to serve as a framework for obtaining a higher resolution physical map of the chromosome.

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JO - Human Molecular Genetics

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ER -

Isolation of yeast artificial chromosome clones from 54 polymorphic loci mapped with high odds on human chromosome 4

Abstract

ASJC Scopus subject areas

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