Isolation of yeast artificial chromosome clones from 54 polymorphic loci mapped with high odds on human chromosome 4

Jlan Bing Fan, Joe Deyoung, Rob Lagacé, R. Andrea Lina, Zhldong Xu, Jeffrey C. Murray, Kenneth H. Buetow, Jean Weissenbach, Richard D. Goold, David R. Cox, Richard M. Myers

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Abstract

We constructed a yeast artificial chromosome (YAC) framework map of human chromosome 4 by screening a YAC library with 63 polymorphic DNA markers located on the chromosome. These genetic markers are from two framework melotic maps that had previously been constructed by two research groups, and are placed on the two maps with odds for their order of 1000: 1 or greater. In addition to Isolating and determining the sizes of 141 YAC clones for 54 of these markers, we combined the two framework melotic maps to produce a single Integrated map. These combined maps and the YAC clones provide a set of extended DNA loci ordered at high odds that can be used to isolate additional polymorphic loci and genes, and to serve as a framework for obtaining a higher resolution physical map of the chromosome.

Original languageEnglish (US)
Pages (from-to)243-246
Number of pages4
JournalHuman Molecular Genetics
Volume3
Issue number2
DOIs
StatePublished - Feb 1 1994

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ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

Cite this

Fan, J. B., Deyoung, J., Lagacé, R., Lina, R. A., Xu, Z., Murray, J. C., Buetow, K. H., Weissenbach, J., Goold, R. D., Cox, D. R., & Myers, R. M. (1994). Isolation of yeast artificial chromosome clones from 54 polymorphic loci mapped with high odds on human chromosome 4. Human Molecular Genetics, 3(2), 243-246. https://doi.org/10.1093/hmg/3.2.243