Isolation of yeast artificial chromosome clones from 54 polymorphic loci mapped with high odds on human chromosome 4

Jian Bing Fan, Joe DeYoung, Rob Lagacé, R. Andrea Lina, Zhidong Xu, Jeffrey C. Murray, Kenneth Buetow, Jean Weissenbach, Richard D. Goold, David R. Cox, Richard M. Myers

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We constructed a yeast artificial chromosome (YAC) framework map of human chromosome 4 by screening a YAC library with 63 polymorphic DNA markers located on the chromosome. These genetic markers are from two framework meiotic maps that had previously been constructed by two research groups, and are placed on the two maps with odds for their order of 1000:1 or greater. In addition to isolating and determining the sizes of 141 YAC clones for 54 of these markers, we combined the two framework meiotic maps to produce a single integrated map. These combined maps and the YAC clones provide a set of extended DNA loci ordered at high odds that can be used to isolate additional polymorphic loci and genes, and to serve as a framework for obtaining a higher resolution physical map of the chromosome.

Original languageEnglish (US)
Pages (from-to)243-246
Number of pages4
JournalHuman Molecular Genetics
Volume3
Issue number2
StatePublished - Feb 1994
Externally publishedYes

Fingerprint

Yeast Artificial Chromosomes
Chromosomes, Human, Pair 4
Odds
Human Chromosomes
Chromosomes
Clone
Yeast
Chromosome
Isolation
Locus
Clone Cells
Genetic Markers
DNA
Human
Screening
Research
Genes
High Resolution
Gene
Framework

ASJC Scopus subject areas

  • Genetics
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Public Health, Environmental and Occupational Health
  • Molecular Biology
  • Genetics(clinical)

Cite this

Fan, J. B., DeYoung, J., Lagacé, R., Lina, R. A., Xu, Z., Murray, J. C., ... Myers, R. M. (1994). Isolation of yeast artificial chromosome clones from 54 polymorphic loci mapped with high odds on human chromosome 4. Human Molecular Genetics, 3(2), 243-246.

Isolation of yeast artificial chromosome clones from 54 polymorphic loci mapped with high odds on human chromosome 4. / Fan, Jian Bing; DeYoung, Joe; Lagacé, Rob; Lina, R. Andrea; Xu, Zhidong; Murray, Jeffrey C.; Buetow, Kenneth; Weissenbach, Jean; Goold, Richard D.; Cox, David R.; Myers, Richard M.

In: Human Molecular Genetics, Vol. 3, No. 2, 02.1994, p. 243-246.

Research output: Contribution to journalArticle

Fan, JB, DeYoung, J, Lagacé, R, Lina, RA, Xu, Z, Murray, JC, Buetow, K, Weissenbach, J, Goold, RD, Cox, DR & Myers, RM 1994, 'Isolation of yeast artificial chromosome clones from 54 polymorphic loci mapped with high odds on human chromosome 4', Human Molecular Genetics, vol. 3, no. 2, pp. 243-246.
Fan, Jian Bing ; DeYoung, Joe ; Lagacé, Rob ; Lina, R. Andrea ; Xu, Zhidong ; Murray, Jeffrey C. ; Buetow, Kenneth ; Weissenbach, Jean ; Goold, Richard D. ; Cox, David R. ; Myers, Richard M. / Isolation of yeast artificial chromosome clones from 54 polymorphic loci mapped with high odds on human chromosome 4. In: Human Molecular Genetics. 1994 ; Vol. 3, No. 2. pp. 243-246.
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