Isolation of a lethal virus from the endangered tiger salamander Ambystoma tigrinum stebbinsi

James K. Jancovich, Elizabeth W. Davidson, J. Frank Morado, Bertram Jacobs, James Collins

Research output: Contribution to journalArticlepeer-review

171 Scopus citations

Abstract

The Sonora tiger salamander Arabystoma tigrinum stebbinsi Lowe is a genetically distinct race restricted to about 30 small ponds in the San Rafael Valley in southern Arizona, USA, which was added recently to the USA Federal List of Endangered Species. Populations of these salamanders periodically experience decimating epizootics. Virus was isolated from diseased salamanders using fish cell cultures, injected into healthy laboratory-reared salamanders, and then reisolated in cell culture. Electron microscopy of thin sections from dying salamanders revealed abundant enveloped and non-enveloped icosahedral virus particles approximately 160 to 180 nm in diameter in the cytoplasm of skin and liver cells and free in the intercellular spaces. This virus, believed to be an iridovirus based on viral morphology and host pathology, was demonstrated to be the primary pathogen in these epizootics, and is the first lethal epizootic virus reported from salamanders. We have named the virus Arabystoma tigrinum Virus (ATV). Hemolytic bacteria were isolated from sick individuals, but we were unable to induce the disease by exposing salamanders to isolated bacteria at concentrations up to 108 ml-1.

Original languageEnglish (US)
Pages (from-to)161-167
Number of pages7
JournalDiseases of Aquatic Organisms
Volume31
Issue number3
DOIs
StatePublished - Dec 30 1997

Keywords

  • Amphibian decline
  • Arabystoma tigrinum stebbinsi
  • Iridovirus
  • Salamander
  • Virus

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science

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