Isolation and structure of pedilstatin from a Republic of Maldives Pedilanthus sp.

George Pettit; Sylvie Ducki; Rui Tan; Roberta S. Gardella; James B. McMahon; Michael R. Boyd; George R. Pettit; Peter M. Blumberg; Nancy E. Lewin; Dennis L. Doubek; Larry P. Tackett; Michael D. Williams

doi:10.1021/np020115b

Isolation and structure of pedilstatin from a Republic of Maldives Pedilanthus sp.

George Pettit, Sylvie Ducki, Rui Tan, Roberta S. Gardella, James B. McMahon, Michael R. Boyd, George R. Pettit, Peter M. Blumberg, Nancy E. Lewin, Dennis L. Doubek, Larry P. Tackett, Michael D. Williams

Molecular Sciences, School of (SMS)

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

A new cancer cell growth inhibitor designated pedilstatin (1) was isolated from a Republic of Maldives Pedilanthus sp. The structure was determined to be 13-Oacetyl-12-O-[2′Z,4′-octadienoyl]-4α-deoxyphorbol on the basis of high-resolution mass spectral and 2D NMR assignments. Pedilstatin was found to significantly inhibit growth of the P388 lymphocytic leukemia cell line with an ED₅₀ of 0.28 μg/mL, to afford, at concentrations of 2-5 μM, protection (to 80%) of human-derived lymphoblastoid CEM-SS cells from infection and cell-killing by HIV-1, and to show inhibition of protein kinase C with a K_i of 620 ± 20 nM.

Original language	English (US)
Pages (from-to)	1262-1265
Number of pages	4
Journal	Journal of Natural Products
Volume	65
Issue number	9
DOIs	https://doi.org/10.1021/np020115b
State	Published - Sep 1 2002

ASJC Scopus subject areas

Analytical Chemistry
Molecular Medicine
Pharmacology
Pharmaceutical Science
Drug Discovery
Complementary and alternative medicine
Organic Chemistry

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title = "Isolation and structure of pedilstatin from a Republic of Maldives Pedilanthus sp.",

abstract = "A new cancer cell growth inhibitor designated pedilstatin (1) was isolated from a Republic of Maldives Pedilanthus sp. The structure was determined to be 13-Oacetyl-12-O-[2′Z,4′-octadienoyl]-4α-deoxyphorbol on the basis of high-resolution mass spectral and 2D NMR assignments. Pedilstatin was found to significantly inhibit growth of the P388 lymphocytic leukemia cell line with an ED50 of 0.28 μg/mL, to afford, at concentrations of 2-5 μM, protection (to 80%) of human-derived lymphoblastoid CEM-SS cells from infection and cell-killing by HIV-1, and to show inhibition of protein kinase C with a Ki of 620 ± 20 nM.",

author = "George Pettit and Sylvie Ducki and Rui Tan and Gardella, {Roberta S.} and McMahon, {James B.} and Boyd, {Michael R.} and Pettit, {George R.} and Blumberg, {Peter M.} and Lewin, {Nancy E.} and Doubek, {Dennis L.} and Tackett, {Larry P.} and Williams, {Michael D.}",

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T1 - Isolation and structure of pedilstatin from a Republic of Maldives Pedilanthus sp.

AU - Pettit, George

AU - Ducki, Sylvie

AU - Tan, Rui

AU - Gardella, Roberta S.

AU - McMahon, James B.

AU - Boyd, Michael R.

AU - Pettit, George R.

AU - Blumberg, Peter M.

AU - Lewin, Nancy E.

AU - Doubek, Dennis L.

AU - Tackett, Larry P.

AU - Williams, Michael D.

PY - 2002/9/1

Y1 - 2002/9/1

N2 - A new cancer cell growth inhibitor designated pedilstatin (1) was isolated from a Republic of Maldives Pedilanthus sp. The structure was determined to be 13-Oacetyl-12-O-[2′Z,4′-octadienoyl]-4α-deoxyphorbol on the basis of high-resolution mass spectral and 2D NMR assignments. Pedilstatin was found to significantly inhibit growth of the P388 lymphocytic leukemia cell line with an ED50 of 0.28 μg/mL, to afford, at concentrations of 2-5 μM, protection (to 80%) of human-derived lymphoblastoid CEM-SS cells from infection and cell-killing by HIV-1, and to show inhibition of protein kinase C with a Ki of 620 ± 20 nM.

AB - A new cancer cell growth inhibitor designated pedilstatin (1) was isolated from a Republic of Maldives Pedilanthus sp. The structure was determined to be 13-Oacetyl-12-O-[2′Z,4′-octadienoyl]-4α-deoxyphorbol on the basis of high-resolution mass spectral and 2D NMR assignments. Pedilstatin was found to significantly inhibit growth of the P388 lymphocytic leukemia cell line with an ED50 of 0.28 μg/mL, to afford, at concentrations of 2-5 μM, protection (to 80%) of human-derived lymphoblastoid CEM-SS cells from infection and cell-killing by HIV-1, and to show inhibition of protein kinase C with a Ki of 620 ± 20 nM.

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JO - Journal of Natural Products

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ER -

Isolation and structure of pedilstatin from a Republic of Maldives Pedilanthus sp.

Abstract

ASJC Scopus subject areas

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