Isolation and structure of axinastatin 4 from the western indian ocean marine sponge Axinella cf. carteri

George Pettit, Feng Gao, Ronald Cerny

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Abstract

The Republic of The Comoros marine sponge Axinella cf. carteri has been found to contain a cell growth inhibitory (P388 lymphocytic leukemia cell line ED50 0.057 μg/ml, and comparable activity against a series of human cancer cell lines) cyclo-heptapeptide named axinastatin 4 (3). The new peptide was obtained in 1.0 × 10-6% yield (6.1 mg) from 600 kg (wet wt) of the sponge. The structure of axinastatin 4 was deduced as cyclo-(Pro-Leu-Thr-Pro-Leu-Trp-Val) by a combination of high field (400 and 500 Mhz) 2D nmr (1H-1H, 1H-13C, HMBC and NOE) and high resolution mass spectral (principally by ms/ms) measurements and subsequent interpretations.

Original languageEnglish (US)
Pages (from-to)711-718
Number of pages8
JournalHeterocycles
Volume35
Issue number2
StatePublished - May 1 1993

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ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmacology
  • Organic Chemistry

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