Isolation and identification of four cytokinins from wheat germ transfer ribonucleic acid

W. J. Burrows, D. J. Armstrong, M. Kaminek, F. Skoog, R. M. Bock, S. M. Hecht, L. G. Dammann, N. J. Leonard, Sidney Hecht

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Abstract

Four ribonucleosides responsible for cytokinin activity in wheat germ tRNA have been isolated and identified as 6-(3-methyl-2-butenylamino)-9-β-D-ribofuranosylpurine, 6-(4-hydroxy-3-methyl-2-butenylamino)-9-β-D-ribofuranosyl-purine, 6-(3-methyl-2-butenylamino)-2-methylthio-9-/3-Dribofuranosylpurine, and 6-(4-hydroxy-3-methyl-2-butenylamino)-2-methylthio-9-β-D- ribofuranosylpurine. tRNA was extracted from defatted wheat germ and was hydrolyzed enzymatically. The ribonucleosides were extracted with ethyl acetate, and fractionated on Sephadex LH-20 columns and on paper. Cytokinin activity in the eluates was followed by the tobacco callus bioassay. The structures of these compounds were assigned on the basis of their Chromatographic properties and ultraviolet and mass spectra, which were identical with those of the corresponding synthetic compounds.

Original languageEnglish (US)
Pages (from-to)1867-1872
Number of pages6
JournalBiochemistry
Volume9
Issue number9
StatePublished - 1970
Externally publishedYes

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Ribonucleosides
Cytokinins
Transfer RNA
Triticum
Tobacco
Bioassay
Bony Callus
Biological Assay
ethyl acetate
N(6)-(delta(2)-isopentenyl)adenine
sephadex

ASJC Scopus subject areas

  • Biochemistry

Cite this

Burrows, W. J., Armstrong, D. J., Kaminek, M., Skoog, F., Bock, R. M., Hecht, S. M., ... Hecht, S. (1970). Isolation and identification of four cytokinins from wheat germ transfer ribonucleic acid. Biochemistry, 9(9), 1867-1872.

Isolation and identification of four cytokinins from wheat germ transfer ribonucleic acid. / Burrows, W. J.; Armstrong, D. J.; Kaminek, M.; Skoog, F.; Bock, R. M.; Hecht, S. M.; Dammann, L. G.; Leonard, N. J.; Hecht, Sidney.

In: Biochemistry, Vol. 9, No. 9, 1970, p. 1867-1872.

Research output: Contribution to journalArticle

Burrows, WJ, Armstrong, DJ, Kaminek, M, Skoog, F, Bock, RM, Hecht, SM, Dammann, LG, Leonard, NJ & Hecht, S 1970, 'Isolation and identification of four cytokinins from wheat germ transfer ribonucleic acid', Biochemistry, vol. 9, no. 9, pp. 1867-1872.
Burrows WJ, Armstrong DJ, Kaminek M, Skoog F, Bock RM, Hecht SM et al. Isolation and identification of four cytokinins from wheat germ transfer ribonucleic acid. Biochemistry. 1970;9(9):1867-1872.
Burrows, W. J. ; Armstrong, D. J. ; Kaminek, M. ; Skoog, F. ; Bock, R. M. ; Hecht, S. M. ; Dammann, L. G. ; Leonard, N. J. ; Hecht, Sidney. / Isolation and identification of four cytokinins from wheat germ transfer ribonucleic acid. In: Biochemistry. 1970 ; Vol. 9, No. 9. pp. 1867-1872.
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