TY - JOUR
T1 - Isolation and enrichment of secreted proteins from filamentous fungi.
AU - Medina, Martha L.
AU - Francisco, Wilson A.
PY - 2008
Y1 - 2008
N2 - Filamentous fungi have been recognized as extraordinary producers of secreted proteins and are known to produce novel proteins and enzymes through dispensable metabolic pathways. Here, methods are described for the isolation and enrichment of samples of secreted proteins from cultures of filamentous fungi for analysis by gel electrophoresis and mass spectrometry techniques. These methods can be readily applied to the study of differential protein expression and secretion and metabolic pathways in filamentous fungi by proteomic approaches.
AB - Filamentous fungi have been recognized as extraordinary producers of secreted proteins and are known to produce novel proteins and enzymes through dispensable metabolic pathways. Here, methods are described for the isolation and enrichment of samples of secreted proteins from cultures of filamentous fungi for analysis by gel electrophoresis and mass spectrometry techniques. These methods can be readily applied to the study of differential protein expression and secretion and metabolic pathways in filamentous fungi by proteomic approaches.
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U2 - 10.1007/978-1-60327-210-0_22
DO - 10.1007/978-1-60327-210-0_22
M3 - Article
C2 - 18369903
AN - SCOPUS:44449085438
SN - 1064-3745
VL - 425
SP - 275
EP - 285
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -