Isolation and enrichment of secreted proteins from filamentous fungi.

Martha L. Medina, Wilson A. Francisco

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Filamentous fungi have been recognized as extraordinary producers of secreted proteins and are known to produce novel proteins and enzymes through dispensable metabolic pathways. Here, methods are described for the isolation and enrichment of samples of secreted proteins from cultures of filamentous fungi for analysis by gel electrophoresis and mass spectrometry techniques. These methods can be readily applied to the study of differential protein expression and secretion and metabolic pathways in filamentous fungi by proteomic approaches.

Original languageEnglish (US)
Pages (from-to)275-285
Number of pages11
JournalMethods in molecular biology (Clifton, N.J.)
Volume425
StatePublished - 2008

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Fungi
Metabolic Networks and Pathways
Proteins
Secretory Pathway
Proteomics
Electrophoresis
Mass Spectrometry
Gels
Enzymes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Isolation and enrichment of secreted proteins from filamentous fungi. / Medina, Martha L.; Francisco, Wilson A.

In: Methods in molecular biology (Clifton, N.J.), Vol. 425, 2008, p. 275-285.

Research output: Contribution to journalArticle

Medina, Martha L. ; Francisco, Wilson A. / Isolation and enrichment of secreted proteins from filamentous fungi. In: Methods in molecular biology (Clifton, N.J.). 2008 ; Vol. 425. pp. 275-285.
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