Isolation and characterization of conditional adherent and non-type 1 fimbriated Salmonella typhimurium mutants

Hank A. Lockman, Roy Curtiss

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Mutations in the genes encoding the type 1 fimbriae of Salmonella typhimurium were isolated by selecting for the deletion of Tn10 inserted adjacent to the chromosomal fim+ genes and screening for the loss of mannose-sensitive haemagglutination (HA) activity. S. typhimurium strains with Tn10 insertions in ahp were hypersensitive to peroxides, and tetracycline-sensitive derivatives of ahp::Tn10 mutants displayed two fim mutant phenotypes. The predominant class of fim mutants did not synthesize type 1 fimbriae. A second type of fim mutant synthesized type 1 fimbriae and exhibited a conditional lipoic acid requirement for HA. A fim-lip conditional mutant synthesized type 1 fimbriae when grown in Mueller-Hinton broth but the haemagglutinating activity of the fimbriae was dependent upon the addition of lipoic acid to the growth medium. Independently isolated lip mutations did not demonstrate a similar pleiotropic effect on HA. Western blots of fimbriae extracted from a fim-lip conditional mutant that was grown under permissive and restrictive conditions indicated the presence of 33 and 36.6 KDa proteins in HA+ fimbriae that were absent in HA- fimbriae. The HA+ phenotype of both conditional and non-fimbriated mutants was restored by transformation with cloned genes encoding S. typhimurium type 1 fimbriae.

Original languageEnglish (US)
Pages (from-to)933-945
Number of pages13
JournalMolecular Microbiology
Volume6
Issue number7
StatePublished - Apr 1992
Externally publishedYes

Fingerprint

Hemagglutination
Salmonella typhimurium
Lip
Thioctic Acid
Genes
Phenotype
Mutation
Peroxides
Mannose
Tetracycline
Western Blotting
Growth
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

Isolation and characterization of conditional adherent and non-type 1 fimbriated Salmonella typhimurium mutants. / Lockman, Hank A.; Curtiss, Roy.

In: Molecular Microbiology, Vol. 6, No. 7, 04.1992, p. 933-945.

Research output: Contribution to journalArticle

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