Isolation and characterization of complementary deoxyribonucleic acid complementary to the highly abundant class of poly(adenylic acid)-containing ribonucleic acid from oocytes of Drosophila melanogaster

Clarice M. Weide, Walter S. Vincent, Elliott S. Goldstein

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The complementary deoxyribonucleic acid (cDNA) complementary to the highly abundant class of poly(adenylic acid)-containing ribonucleic acid [poly(A+) RNA] from Drosophila malanogaster oocytes has been isolated and characterized. Analysis of the kinetics of hybridization of this cDNA (cDNAHA) to total poly(A+) RNA of oocytes indicates this class contains ∼86 different sequences. Hybridization kinetics of cDNAHA annealed to poly(A+) RNA from 19-h-old embryos is essentially the same as that of oocyte poly(A+) RNA. This suggests the highly abundant class of poly(A+) RNA persists in approximately the same frequency through early development. Analysis of the hybridization of cDNAHA to genomic DNA suggests that the highly abundant poly(A+) RNA from oocytes is not enriched for transcripts from repetitive sequences of the genome.

Original languageEnglish (US)
Pages (from-to)5092-5098
Number of pages7
JournalBiochemistry
Volume19
Issue number22
StatePublished - 1980

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Adenosine Monophosphate
Drosophila melanogaster
Oocytes
RNA
Messenger RNA
DNA
Kinetics
Nucleic Acid Repetitive Sequences
Drosophila
Embryonic Structures
Genes
Genome

ASJC Scopus subject areas

  • Biochemistry

Cite this

Isolation and characterization of complementary deoxyribonucleic acid complementary to the highly abundant class of poly(adenylic acid)-containing ribonucleic acid from oocytes of Drosophila melanogaster. / Weide, Clarice M.; Vincent, Walter S.; Goldstein, Elliott S.

In: Biochemistry, Vol. 19, No. 22, 1980, p. 5092-5098.

Research output: Contribution to journalArticle

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