Isolation and characterization of chicken lung mesenchymal stromal cells and their susceptibility to avian influenza virus

Mahesh Khatri, Timothy D. O'Brien, Sagar M. Goyal, Jagdev M. Sharma

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

In this study, we isolated and characterized mesenchymal stromal cells (MSCs) from the lungs of 1- to 2-week-old chickens. Microscopically, the cultured cells showed fibroblast-like morphology. Phenotypically these cells expressed CD44, CD90, CD105 and the transcription factor PouV, which has been shown to be critical for stem cell self-renewal and pluripotency. The multipotency of chicken MSCs was demonstrated by their ability to undergo adipogenic and osteogenic differentiation. Like chicken bone marrow MSCs and mammalian MSCs, chicken lung MSCs had immunoregulatory activity and profoundly suppressed the proliferative capacity of T cells in response to a mitogenic stimulus. Next, we examined the susceptibility of these cells to H1N1 and H9N5 avian influenza (AI) viruses. The lung MSCs were shown to express known influenza virus alpha-2,3 and alpha-2,6 sialic acid receptors and to support replication of both the avian H1N1 and avian H9N5 influenza strains. Viral infection of MSCs resulted in cell lysis and cytokine and chemokine production. Further characterization of lung MSCs in chicken and other mammalian species may help in understanding the pathogenesis of infectious and non-infectious lung diseases and the mechanisms of lung injury repair.

Original languageEnglish (US)
Pages (from-to)474-479
Number of pages6
JournalDevelopmental and Comparative Immunology
Volume34
Issue number4
DOIs
StatePublished - Apr 2010
Externally publishedYes

Fingerprint

Influenza in Birds
Orthomyxoviridae
Mesenchymal Stromal Cells
Chickens
Lung
Lung Injury
Virus Diseases
Chemokines
Lung Diseases
Cultured Cells
Transcription Factors
Fibroblasts
Cytokines
T-Lymphocytes

Keywords

  • Avian influenza virus
  • Immunoregulation
  • Mesenchymal stromal cells
  • Mesenchymal stromal cells differentiation
  • Tissue mesenchymal stromal cells

ASJC Scopus subject areas

  • Developmental Biology
  • Immunology

Cite this

Isolation and characterization of chicken lung mesenchymal stromal cells and their susceptibility to avian influenza virus. / Khatri, Mahesh; O'Brien, Timothy D.; Goyal, Sagar M.; Sharma, Jagdev M.

In: Developmental and Comparative Immunology, Vol. 34, No. 4, 04.2010, p. 474-479.

Research output: Contribution to journalArticle

Khatri, Mahesh ; O'Brien, Timothy D. ; Goyal, Sagar M. ; Sharma, Jagdev M. / Isolation and characterization of chicken lung mesenchymal stromal cells and their susceptibility to avian influenza virus. In: Developmental and Comparative Immunology. 2010 ; Vol. 34, No. 4. pp. 474-479.
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