Isolation and characterization of cDNA complementary to transient maternal poly(A)+ RNA from the Drosophila oocyte

Elliott S. Goldstein, Charles G. Arthur

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

cDNA complementary to total oocyte poly(A)+ RNA from Drosophila melanogaster was enriched for sequences complementary to transient maternal sequences; that is, those sequences which disappear from the oocyte during subsequent. A seven- to ten-fold enrichment factor was obtained, from 5.3% to about 50% of the total cDNA. Kinetic analysis of this enriched fraction indicates that the transient maternal sequences include 44 ± 14 different sequences.

Original languageEnglish (US)
Pages (from-to)265-274
Number of pages10
JournalBBA Section Nucleic Acids And Protein Synthesis
Volume565
Issue number2
DOIs
StatePublished - Dec 17 1979

Keywords

  • (Drosophila)
  • Complementary DNA
  • Embryogenesis
  • Oocyte
  • Poly(A) RNA

ASJC Scopus subject areas

  • Medicine(all)

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