Abstract

A novel approach to molecular separations is investigated using a technique termed droplet-based isoelectric focusing. Drops are manipulated discretely on a superhydrophobic surface, subjected to low voltages for isoelectric focusing, and split-resulting in a preparative separation. A universal indicator dye demonstrates the generation of stable, reversible pH gradients (3-10) in ampholyte buffers, and these gradients lead to protein focusing within the drop length. Focusing was visually characterized, spectroscopically verified, and assessed quantitatively by noninvasive light scattering measurements. It was found to correlate with a quantitative model based on 1D steady-state theory. This work illustrates that molecular separations can be deployed within a single open drop, and the differential fractions can be separated into new discrete liquid elements.

Original languageEnglish (US)
Pages (from-to)494-498
Number of pages5
JournalLangmuir
Volume27
Issue number1
DOIs
StatePublished - Jan 4 2011

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Buffers
gradients
Light scattering
low voltage
light scattering
Coloring Agents
Dyes
buffers
dyes
proteins
Proteins
Isoelectric Focusing
Liquids
Electric potential
liquids

ASJC Scopus subject areas

  • Electrochemistry
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Materials Science(all)
  • Spectroscopy

Cite this

Isoelectric focusing in a drop. / Weiss, Noah G.; Hayes, Mark; Garcia, Antonio; Ansari, Rafat R.

In: Langmuir, Vol. 27, No. 1, 04.01.2011, p. 494-498.

Research output: Contribution to journalArticle

Weiss, NG, Hayes, M, Garcia, A & Ansari, RR 2011, 'Isoelectric focusing in a drop', Langmuir, vol. 27, no. 1, pp. 494-498. https://doi.org/10.1021/la104085t
Weiss, Noah G. ; Hayes, Mark ; Garcia, Antonio ; Ansari, Rafat R. / Isoelectric focusing in a drop. In: Langmuir. 2011 ; Vol. 27, No. 1. pp. 494-498.
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