Is otospiralin inner ear specific? Evidence for its expression in mouse brain

Boris DeCourt, Dean Hillman, Yohan Bouleau, Didier Dulon, Aziz Hafidi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The small protein otospiralin has initially been identified as an inner ear specific molecule. However, compelling evidence from high throughput sequencing projects suggested that otospiralin is likely expressed in the central nervous system. Here, we tested this hypothesis using a combination of molecular biology, immunological, and histological techniques, and found that otospiralin is expressed in numerous regions of the central nervous system in mouse. In situ hybridization and immunohistochemistry revealed that otospiralin is widely expressed in neuronal cell bodies and glia. Ultrastructural observations in the cerebral cortex located the small protein in close proximity to membranous organelles in perikarya, the inner face of post-synaptic neuronal membranes, and in astrocytic processes. These results are in agreement with the predicted structure of the protein which revealed a single N-terminal transmembrane helix domain followed by a C-terminus cytosolic tail. Interestingly, 2 weeks after a mechanical trauma in the cerebral cortex, otospiralin expression increased in reactive astrocytes located within the vicinity of the site of injury, but not in neurons. Collectively, our observations suggest that otospiralin is possibly involved in signaling pathways, and could play a role in repair mechanisms subsequent to an injury in the central nervous system.

Original languageEnglish (US)
Pages (from-to)87-96
Number of pages10
JournalInternational Journal of Developmental Neuroscience
Volume27
Issue number1
DOIs
StatePublished - Feb 1 2009
Externally publishedYes

Fingerprint

Inner Ear
Central Nervous System
Cerebral Cortex
Wounds and Injuries
Brain
Immunologic Techniques
Histological Techniques
Synaptic Membranes
Proteins
Neuroglia
Astrocytes
Organelles
In Situ Hybridization
Tail
Molecular Biology
Immunohistochemistry
Neurons

Keywords

  • Astrocyte
  • Axonal regeneration
  • Brain
  • Electron microscopy
  • In situ hybridization
  • Lesion

ASJC Scopus subject areas

  • Developmental Neuroscience
  • Developmental Biology

Cite this

Is otospiralin inner ear specific? Evidence for its expression in mouse brain. / DeCourt, Boris; Hillman, Dean; Bouleau, Yohan; Dulon, Didier; Hafidi, Aziz.

In: International Journal of Developmental Neuroscience, Vol. 27, No. 1, 01.02.2009, p. 87-96.

Research output: Contribution to journalArticle

DeCourt, Boris ; Hillman, Dean ; Bouleau, Yohan ; Dulon, Didier ; Hafidi, Aziz. / Is otospiralin inner ear specific? Evidence for its expression in mouse brain. In: International Journal of Developmental Neuroscience. 2009 ; Vol. 27, No. 1. pp. 87-96.
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