Abstract

We present results showing that silicon substrates can be used as a universal platform for recording the electrical activity of ion channels inserted into suspended bilayer membranes. The bilayers span 150 μm apertures etched into silicon substrates using standard microelectronics processing techniques. The silicon is oxidized, patterned with a 75 μm thick SU-8 epoxy resist and then coated with a thin layer of polytetrafluoroethylene rendering the surface hydrophobic. Reversible Ag/AgCl electrodes are integrated around the circumference of the opening and provide long-term stable measurements of the ion channel currents. Characteristic measurements of OmpF porin ion channel protein in phospholipid bilayers and α-hemolysin toxin protein in triblock copolymer layers were made. Long-term measurements showed that ion channel activity could be recorded 22 hours after initial formation of a lipid bilayer.

Original languageEnglish (US)
Journale-Journal of Surface Science and Nanotechnology
Volume3
DOIs
StatePublished - Jun 8 2005

Keywords

  • Biological molecules -proteins
  • Biomedical engineering
  • Ion channel
  • Lipid bilayer membrane
  • Microfabrication

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Condensed Matter Physics
  • Mechanics of Materials
  • Surfaces and Interfaces
  • Surfaces, Coatings and Films

Fingerprint Dive into the research topics of 'Ion channels on silicon'. Together they form a unique fingerprint.

Cite this