Abstract

We are building a biosensor based on ion channels inserted into lipid bilayers that are suspended across an aperture in silicon. The process flow only involves conventional optical lithography and deep Si reactive ion etching to create micromachined apertures in a silicon wafer. In order to provide surface properties for lipid bilayer attachment that are similar to those of the fluorocarbon films that are currently used, we coated the silicon surface with a fluoropolymer using plasma-assisted chemical vapor deposition. When compared with the surface treatment methods using self-assembled monolayers of fluorocarbon chemicals, this novel approach towards modifying the wettability of a silicon dioxide surface provides an easy and fast method for subsequent lipid bilayer formation. Current-Voltage measurements on OmpF ion channels incorporated into these membranes show the voltage dependent gating action expected from a working porin ion channel.

Original languageEnglish (US)
Title of host publicationMaterials Research Society Symposium Proceedings
EditorsJ. Liu, D. McIlroy, L. Merhari, J.P. Pendry, J.T. Borenstein, P. Grodzinski, L.P. Lee, Z.L. Wang
Pages163-170
Number of pages8
Volume820
StatePublished - 2004
EventNanoengineered Assemblies and Advanced Micro/Nanosystems - San Francisco, CA, United States
Duration: Apr 13 2004Apr 16 2004

Other

OtherNanoengineered Assemblies and Advanced Micro/Nanosystems
CountryUnited States
CitySan Francisco, CA
Period4/13/044/16/04

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials

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