Involvement of the HtrA family of proteases in the protection of the cyanobacterium Synechocystis PCC 6803 from light stress and in the repair of photosystem II

Paulo Silva, Young Jun Choi, Hanadi A G Hassan, Peter J. Nixon, C. Howe, T. Hundal, B. Andersson, Petra Fromme, J. Komenda

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Photosystem II (PSII) is prone to irreversible light-induced damage, with the D1 polypeptide a major target. Repair processes operate in the cell to replace a damaged D1 subunit within the complex with a newly synthesized copy. As yet, the molecular details of PSII repair are relatively obscure despite the critical importance of this process for maintaining PSII activity and cell viability. We are using the cyanobacterium Synechocystis sp. PCC 6803 to identify the various proteases and chaperones involved in D1 turnover in vivo. Two families of proteases are being studied: the FtsH family (four members) of Zn2+-activated nucleotide-dependent proteases; and the HtrA (or DegP) family (three members) of serine-type proteases. In this paper, we report the results of our studies on a triple mutant in which all three copies of the htrA gene family have been inactivated. Growth of the mutant on agar plates was inhibited at high light intensities, especially in the presence of glucose. Oxygen evolution measurements indicated that, under conditions of high light, the rate of synthesis of functional PSII was less in the mutant than in the wild-type. Immunoblotting experiments conducted on cells blocked in protein synthesis further indicated that degradation of D1 was slowed in the mutant. Overall, our observations indicate that the HtrA family of proteases are involved in the resistance of Synechocystis 6803 to light stress and play a part, either directly or indirectly, in the repair of PSII in vivo.

Original languageEnglish (US)
Pages (from-to)1461-1468
Number of pages8
JournalPhilosophical Transactions of the Royal Society B: Biological Sciences
Volume357
Issue number1426
DOIs
StatePublished - Oct 29 2002
Externally publishedYes

Fingerprint

Synechocystis
Photosystem II Protein Complex
Cyanobacteria
photosystem II
repair
cyanobacterium
Repair
Peptide Hydrolases
proteinases
Light
Synechocystis sp. PCC 6803
mutants
High intensity light
Serine Proteases
immunoblotting
Immunoblotting
light intensity
agar
serine
Agar

Keywords

  • D1 protein repair cycle
  • DegP
  • HtrA
  • Photoinhibition
  • Photosystem II

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Agricultural and Biological Sciences (miscellaneous)

Cite this

Involvement of the HtrA family of proteases in the protection of the cyanobacterium Synechocystis PCC 6803 from light stress and in the repair of photosystem II. / Silva, Paulo; Choi, Young Jun; Hassan, Hanadi A G; Nixon, Peter J.; Howe, C.; Hundal, T.; Andersson, B.; Fromme, Petra; Komenda, J.

In: Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 357, No. 1426, 29.10.2002, p. 1461-1468.

Research output: Contribution to journalArticle

Silva, Paulo ; Choi, Young Jun ; Hassan, Hanadi A G ; Nixon, Peter J. ; Howe, C. ; Hundal, T. ; Andersson, B. ; Fromme, Petra ; Komenda, J. / Involvement of the HtrA family of proteases in the protection of the cyanobacterium Synechocystis PCC 6803 from light stress and in the repair of photosystem II. In: Philosophical Transactions of the Royal Society B: Biological Sciences. 2002 ; Vol. 357, No. 1426. pp. 1461-1468.
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AU - Hassan, Hanadi A G

AU - Nixon, Peter J.

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AU - Fromme, Petra

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