Investigation of thiourea activated polyglutaraldehyde with bound Ag(I) or Pt(II) as an alternative to avidin for immobilizing biotin conjugates

D. H. Kim, D. R. Miles, J. W. Knotts, Antonio Garcia

Research output: Contribution to journalArticle

Abstract

Pre-polymerized glutaraldehyde covalently linked to thiourea has been synthesized as a soluble polymer for immobilizing Ag(I) and Pt(II) and it has also been used for activating a polyacrylamide gel filtration media. The modified gel filtration media (Bio-Gel P-200) has a high capacity for Ag(I) (20 μmol/ml) and Pt(II) (8 μmol/ml) and has been shown to be stable and useful even in the presence of relatively high chloride (up to 1 M NaCl) and phosphate concentrations (0.25 M). The soluble polymer can have aAg(I) capacity of between 2-11 mmol/g. Bio-Gel P-200 modified using glutaraldehyde/thiourea and in the Ag(I) and Pt(II) form selectively binds biotinylated BSA (b-BSA) over BSA. Using the Ag(I) form of the gel at pH 4.8 (0.05 M phosphate) only b-BSA binds and 30% can be eluted using 0.15 M NaCl, while no BSA binds to the column under these conditions. For the Pt(II) form of Bio-Gel P-200 at pH 4.8, none of the applied BSA binds to the modified resin while 40% of b-BSA does bind.

Original languageEnglish (US)
Pages (from-to)555-563
Number of pages9
JournalArtificial Cells, Blood Substitutes, and Immobilization Biotechnology
Volume23
Issue number5
Publication statusPublished - 1995

    Fingerprint

ASJC Scopus subject areas

  • Biotechnology
  • Biomedical Engineering
  • Biomaterials
  • Hematology

Cite this