Investigation of thiourea activated polyglutaraldehyde with bound Ag(I) or Pt(II) as an alternative to avidin for immobilizing biotin conjugates

D. H. Kim, D. Miles, Antonio Garcia

Research output: Contribution to journalArticle

Abstract

A method was developed for immobilizing Ag(I) and Pt(II) onto a gel filtration media using polymerized glutaraldehyde activated with thiourea. The resultant gel resin has high capacity for immobilizing biotin conjugates and was shown to be stable and useful even in the presence of relatively high chloride and phosphate concentrations.

Original languageEnglish (US)
JournalArtificial Cells, Blood Substitutes, and Immobilization Biotechnology
Volume22
Issue number5
StatePublished - Nov 1994

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Thiourea
Avidin
Thioureas
Glutaral
Biotin
Gel Chromatography
Chlorides
Gels
Phosphates
Resins
poly(glutaraldehyde)

ASJC Scopus subject areas

  • Biotechnology
  • Biomedical Engineering
  • Biomaterials
  • Hematology

Cite this

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abstract = "A method was developed for immobilizing Ag(I) and Pt(II) onto a gel filtration media using polymerized glutaraldehyde activated with thiourea. The resultant gel resin has high capacity for immobilizing biotin conjugates and was shown to be stable and useful even in the presence of relatively high chloride and phosphate concentrations.",
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