Abstract
This work presents a technique termed as "electrophoretic exclusion" that is capable of differentiation and concentration of proteins in bulk solution. In this method, a hydrodynamic flow is countered by the electrophoretic velocity to prevent a species from entering into a channel. The separation can be controlled by changing the flow rate or applied electric potential in order to exclude a certain species selectively while allowing others to pass through the capillary. The exclusion of various proteins is investigated using a flow-injection regime of the method. Concentration of myoglobin of up to 1200 times the background concentration in 60 s was demonstrated. Additionally, negatively charged myoglobin was separated from a solution containing negatively charged allophycocyanin. Cationic cytochrome c was also differentiated from a solution with allophycocyanin. The ability to differentially transport species in bulk solution enables parallel and serial separation modes not available with other separations schemes.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 368-373 |
| Number of pages | 6 |
| Journal | Analytical Chemistry |
| Volume | 83 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 2011 |
ASJC Scopus subject areas
- Analytical Chemistry