Investigation of electrophoretic exclusion method for the concentration and differentiation of proteins

Michelle M. Meighan, Jared Vasquez, Luke Dziubcynski, Sarah Hews, Mark Hayes

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

This work presents a technique termed as "electrophoretic exclusion" that is capable of differentiation and concentration of proteins in bulk solution. In this method, a hydrodynamic flow is countered by the electrophoretic velocity to prevent a species from entering into a channel. The separation can be controlled by changing the flow rate or applied electric potential in order to exclude a certain species selectively while allowing others to pass through the capillary. The exclusion of various proteins is investigated using a flow-injection regime of the method. Concentration of myoglobin of up to 1200 times the background concentration in 60 s was demonstrated. Additionally, negatively charged myoglobin was separated from a solution containing negatively charged allophycocyanin. Cationic cytochrome c was also differentiated from a solution with allophycocyanin. The ability to differentially transport species in bulk solution enables parallel and serial separation modes not available with other separations schemes.

Original languageEnglish (US)
Pages (from-to)368-373
Number of pages6
JournalAnalytical Chemistry
Volume83
Issue number1
DOIs
StatePublished - Jan 1 2011

Fingerprint

Myoglobin
Proteins
Cytochromes c
Hydrodynamics
Flow rate
Electric potential
allophycocyanin

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Investigation of electrophoretic exclusion method for the concentration and differentiation of proteins. / Meighan, Michelle M.; Vasquez, Jared; Dziubcynski, Luke; Hews, Sarah; Hayes, Mark.

In: Analytical Chemistry, Vol. 83, No. 1, 01.01.2011, p. 368-373.

Research output: Contribution to journalArticle

Meighan, Michelle M. ; Vasquez, Jared ; Dziubcynski, Luke ; Hews, Sarah ; Hayes, Mark. / Investigation of electrophoretic exclusion method for the concentration and differentiation of proteins. In: Analytical Chemistry. 2011 ; Vol. 83, No. 1. pp. 368-373.
@article{185732cd01784c8db4e392541638a3e4,
title = "Investigation of electrophoretic exclusion method for the concentration and differentiation of proteins",
abstract = "This work presents a technique termed as {"}electrophoretic exclusion{"} that is capable of differentiation and concentration of proteins in bulk solution. In this method, a hydrodynamic flow is countered by the electrophoretic velocity to prevent a species from entering into a channel. The separation can be controlled by changing the flow rate or applied electric potential in order to exclude a certain species selectively while allowing others to pass through the capillary. The exclusion of various proteins is investigated using a flow-injection regime of the method. Concentration of myoglobin of up to 1200 times the background concentration in 60 s was demonstrated. Additionally, negatively charged myoglobin was separated from a solution containing negatively charged allophycocyanin. Cationic cytochrome c was also differentiated from a solution with allophycocyanin. The ability to differentially transport species in bulk solution enables parallel and serial separation modes not available with other separations schemes.",
author = "Meighan, {Michelle M.} and Jared Vasquez and Luke Dziubcynski and Sarah Hews and Mark Hayes",
year = "2011",
month = "1",
day = "1",
doi = "10.1021/ac1025495",
language = "English (US)",
volume = "83",
pages = "368--373",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "1",

}

TY - JOUR

T1 - Investigation of electrophoretic exclusion method for the concentration and differentiation of proteins

AU - Meighan, Michelle M.

AU - Vasquez, Jared

AU - Dziubcynski, Luke

AU - Hews, Sarah

AU - Hayes, Mark

PY - 2011/1/1

Y1 - 2011/1/1

N2 - This work presents a technique termed as "electrophoretic exclusion" that is capable of differentiation and concentration of proteins in bulk solution. In this method, a hydrodynamic flow is countered by the electrophoretic velocity to prevent a species from entering into a channel. The separation can be controlled by changing the flow rate or applied electric potential in order to exclude a certain species selectively while allowing others to pass through the capillary. The exclusion of various proteins is investigated using a flow-injection regime of the method. Concentration of myoglobin of up to 1200 times the background concentration in 60 s was demonstrated. Additionally, negatively charged myoglobin was separated from a solution containing negatively charged allophycocyanin. Cationic cytochrome c was also differentiated from a solution with allophycocyanin. The ability to differentially transport species in bulk solution enables parallel and serial separation modes not available with other separations schemes.

AB - This work presents a technique termed as "electrophoretic exclusion" that is capable of differentiation and concentration of proteins in bulk solution. In this method, a hydrodynamic flow is countered by the electrophoretic velocity to prevent a species from entering into a channel. The separation can be controlled by changing the flow rate or applied electric potential in order to exclude a certain species selectively while allowing others to pass through the capillary. The exclusion of various proteins is investigated using a flow-injection regime of the method. Concentration of myoglobin of up to 1200 times the background concentration in 60 s was demonstrated. Additionally, negatively charged myoglobin was separated from a solution containing negatively charged allophycocyanin. Cationic cytochrome c was also differentiated from a solution with allophycocyanin. The ability to differentially transport species in bulk solution enables parallel and serial separation modes not available with other separations schemes.

UR - http://www.scopus.com/inward/record.url?scp=78650777051&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78650777051&partnerID=8YFLogxK

U2 - 10.1021/ac1025495

DO - 10.1021/ac1025495

M3 - Article

VL - 83

SP - 368

EP - 373

JO - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 1

ER -