Investigation of electrophoretic exclusion method for the concentration and differentiation of proteins

Michelle M. Meighan, Jared Vasquez, Luke Dziubcynski, Sarah Hews, Mark Hayes

14 Scopus citations

Abstract

This work presents a technique termed as "electrophoretic exclusion" that is capable of differentiation and concentration of proteins in bulk solution. In this method, a hydrodynamic flow is countered by the electrophoretic velocity to prevent a species from entering into a channel. The separation can be controlled by changing the flow rate or applied electric potential in order to exclude a certain species selectively while allowing others to pass through the capillary. The exclusion of various proteins is investigated using a flow-injection regime of the method. Concentration of myoglobin of up to 1200 times the background concentration in 60 s was demonstrated. Additionally, negatively charged myoglobin was separated from a solution containing negatively charged allophycocyanin. Cationic cytochrome c was also differentiated from a solution with allophycocyanin. The ability to differentially transport species in bulk solution enables parallel and serial separation modes not available with other separations schemes.

Original languageEnglish (US)
Pages (from-to)368-373
Number of pages6
JournalAnalytical Chemistry
Volume83
Issue number1
DOIs
Publication statusPublished - Jan 1 2011

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