Intranasal immunogenicity of a Δcya Δcrp-pab A mutant of Salmonella enterica serotype Typhimurium for the horse

Abhineet S. Sheoran, John F. Timoney, Steven A. Tinge, Preeti Sundaram, Roy Curtiss

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The aim of this study was to investigate the intranasal immunogenicity for the horse of a Δcya Δcrp-pab A mutant (MGN-707) of Salmonella enterica serotype Typhimurium (S. typhimurium). MGN-707 caused no sign of disease, was not detected in feces and a single administration induced strong Salmonella-specific serum and nasal mucosal antibody responses. All ponies had made strong salmonella specific serum IgGa, IgGb, IgA and IgM antibody responses by day 25 after the first immunization. IgM responses to salmonella lipopolysaccharide (LPS) were short lived whereas salmonella specific serum IgGa and IgGb persisted at high levels in all ponies until 83 and 140 days, respectively. Specific nasal mucosal antibody responses dominated by IgA and IgM were evident by day 25 in all ponies except one in which only specific IgGa and IgGb were evident. Specific nasal mucosal IgA persisted in most ponies until day 69. A second immunization on day 140 boosted antibody responses, and stimulated a strong nasal mucosal IgA response in the pony that failed to make an IgA response after primary immunization. At the termination of the experiment, IgA and IgGb dominated jejunal antibody responses whereas vaginal responses were mainly IgA. The latter response unequivocally confirms the existence of a common mucosal immune system in equids. The results indicate that a S. typhimurium Δcya Δcrp-pab A mutant has potential as an intranasal vaccine against salmonellosis in the horse.

Original languageEnglish (US)
Pages (from-to)3787-3795
Number of pages9
JournalVaccine
Volume19
Issue number27
DOIs
StatePublished - Jun 14 2001
Externally publishedYes

Fingerprint

Salmonella enterica
Salmonella Typhimurium
Immunoglobulin A
Horses
immune response
horses
Antibody Formation
mutants
Nose
Salmonella
antibodies
Immunoglobulin M
Immunization
immunization
Salmonella Vaccines
Serum
salmonellosis
Serogroup
Feces
lipopolysaccharides

Keywords

  • Antibody isotype
  • Horse
  • Intranasal
  • Nasal mucosal
  • Salmonella enterica serotype Typhimurium

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

Intranasal immunogenicity of a Δcya Δcrp-pab A mutant of Salmonella enterica serotype Typhimurium for the horse. / Sheoran, Abhineet S.; Timoney, John F.; Tinge, Steven A.; Sundaram, Preeti; Curtiss, Roy.

In: Vaccine, Vol. 19, No. 27, 14.06.2001, p. 3787-3795.

Research output: Contribution to journalArticle

Sheoran, Abhineet S. ; Timoney, John F. ; Tinge, Steven A. ; Sundaram, Preeti ; Curtiss, Roy. / Intranasal immunogenicity of a Δcya Δcrp-pab A mutant of Salmonella enterica serotype Typhimurium for the horse. In: Vaccine. 2001 ; Vol. 19, No. 27. pp. 3787-3795.
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