Intraluminal oxygenation ameliorates ischemia/reperfusion-induced gut mucosal hyperpermeability in pigs

A. Salzman, P. S. Wollert, H. Wang, M. J. Menconi, M. E. Youssef, Carolyn Compton, M. P. Fink

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

We tested the hypothesis that intraluminal oxygenation can prevent increased gut mucosal permeability to a hydrophilic solute caused by mesenteric ischemia/reperfusion (I/R). Studies were performed using pentobarbital-anesthetized immature pigs. Paired 20 cm segments of ileum were perfused intraluminally at 200 ml/hr with either nitrogenated or oxygenated 37°C Ringer's lactate. Beginning at T = -90 min, fluorescein disodium acetate was infused intravenously (12.5 mg/kg bolus, then 12.5 mg/kg/hr). Permeability was assessed by calculating the plasma-to-lumen clearance of fluorescein. Five pigs were subjected to complete mesenteric ischemia (T = 0- 120 min) followed by reperfusion (T = 120-270 min). In nitrogenated segments, I/R resulted in a 1015 ± 195% increase in fluorescein clearance (P < 0.01 vs baseline). In contrast, the increase in fluorescein clearance induced by I/R in oxygenated segments was only 264 ± 63% (P < 0.01 vs baseline). Intraluminal oxygenation afforded significant (P < 0.01) protection against I/R-induced mucosal hyperpermeability at all time points from T = 150-270 min. Histologic sections revealed complete villous epithelial denudation in nitrogenated segments, whereas the mucosal epithelium was largely intact in oxygenated segments. After reperfusion, mucosal ATP levels normalized in oxygenated segments, but there was only partial recovery of ATP levels in nitrogenated segments. These data indicate that intraluminal oxygenation ameliorates mucosal hyperpermeability induced by I/R.

Original languageEnglish (US)
Pages (from-to)37-46
Number of pages10
JournalCirculatory Shock
Volume40
Issue number1
StatePublished - 1993
Externally publishedYes

Fingerprint

Reperfusion
Swine
Ischemia
Fluorescein
Permeability
Adenosine Triphosphate
Pentobarbital
Ileum
Acetates
Epithelium
Mesenteric Ischemia

Keywords

  • ATP
  • fluorescein
  • mucosa
  • oxygen
  • perfusion
  • permeability

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Salzman, A., Wollert, P. S., Wang, H., Menconi, M. J., Youssef, M. E., Compton, C., & Fink, M. P. (1993). Intraluminal oxygenation ameliorates ischemia/reperfusion-induced gut mucosal hyperpermeability in pigs. Circulatory Shock, 40(1), 37-46.

Intraluminal oxygenation ameliorates ischemia/reperfusion-induced gut mucosal hyperpermeability in pigs. / Salzman, A.; Wollert, P. S.; Wang, H.; Menconi, M. J.; Youssef, M. E.; Compton, Carolyn; Fink, M. P.

In: Circulatory Shock, Vol. 40, No. 1, 1993, p. 37-46.

Research output: Contribution to journalArticle

Salzman, A, Wollert, PS, Wang, H, Menconi, MJ, Youssef, ME, Compton, C & Fink, MP 1993, 'Intraluminal oxygenation ameliorates ischemia/reperfusion-induced gut mucosal hyperpermeability in pigs', Circulatory Shock, vol. 40, no. 1, pp. 37-46.
Salzman, A. ; Wollert, P. S. ; Wang, H. ; Menconi, M. J. ; Youssef, M. E. ; Compton, Carolyn ; Fink, M. P. / Intraluminal oxygenation ameliorates ischemia/reperfusion-induced gut mucosal hyperpermeability in pigs. In: Circulatory Shock. 1993 ; Vol. 40, No. 1. pp. 37-46.
@article{2918cf36695141bebb208eb36dee9cb4,
title = "Intraluminal oxygenation ameliorates ischemia/reperfusion-induced gut mucosal hyperpermeability in pigs",
abstract = "We tested the hypothesis that intraluminal oxygenation can prevent increased gut mucosal permeability to a hydrophilic solute caused by mesenteric ischemia/reperfusion (I/R). Studies were performed using pentobarbital-anesthetized immature pigs. Paired 20 cm segments of ileum were perfused intraluminally at 200 ml/hr with either nitrogenated or oxygenated 37°C Ringer's lactate. Beginning at T = -90 min, fluorescein disodium acetate was infused intravenously (12.5 mg/kg bolus, then 12.5 mg/kg/hr). Permeability was assessed by calculating the plasma-to-lumen clearance of fluorescein. Five pigs were subjected to complete mesenteric ischemia (T = 0- 120 min) followed by reperfusion (T = 120-270 min). In nitrogenated segments, I/R resulted in a 1015 ± 195{\%} increase in fluorescein clearance (P < 0.01 vs baseline). In contrast, the increase in fluorescein clearance induced by I/R in oxygenated segments was only 264 ± 63{\%} (P < 0.01 vs baseline). Intraluminal oxygenation afforded significant (P < 0.01) protection against I/R-induced mucosal hyperpermeability at all time points from T = 150-270 min. Histologic sections revealed complete villous epithelial denudation in nitrogenated segments, whereas the mucosal epithelium was largely intact in oxygenated segments. After reperfusion, mucosal ATP levels normalized in oxygenated segments, but there was only partial recovery of ATP levels in nitrogenated segments. These data indicate that intraluminal oxygenation ameliorates mucosal hyperpermeability induced by I/R.",
keywords = "ATP, fluorescein, mucosa, oxygen, perfusion, permeability",
author = "A. Salzman and Wollert, {P. S.} and H. Wang and Menconi, {M. J.} and Youssef, {M. E.} and Carolyn Compton and Fink, {M. P.}",
year = "1993",
language = "English (US)",
volume = "40",
pages = "37--46",
journal = "Journal of inflammation",
issn = "1078-7852",
publisher = "BioMed Central",
number = "1",

}

TY - JOUR

T1 - Intraluminal oxygenation ameliorates ischemia/reperfusion-induced gut mucosal hyperpermeability in pigs

AU - Salzman, A.

AU - Wollert, P. S.

AU - Wang, H.

AU - Menconi, M. J.

AU - Youssef, M. E.

AU - Compton, Carolyn

AU - Fink, M. P.

PY - 1993

Y1 - 1993

N2 - We tested the hypothesis that intraluminal oxygenation can prevent increased gut mucosal permeability to a hydrophilic solute caused by mesenteric ischemia/reperfusion (I/R). Studies were performed using pentobarbital-anesthetized immature pigs. Paired 20 cm segments of ileum were perfused intraluminally at 200 ml/hr with either nitrogenated or oxygenated 37°C Ringer's lactate. Beginning at T = -90 min, fluorescein disodium acetate was infused intravenously (12.5 mg/kg bolus, then 12.5 mg/kg/hr). Permeability was assessed by calculating the plasma-to-lumen clearance of fluorescein. Five pigs were subjected to complete mesenteric ischemia (T = 0- 120 min) followed by reperfusion (T = 120-270 min). In nitrogenated segments, I/R resulted in a 1015 ± 195% increase in fluorescein clearance (P < 0.01 vs baseline). In contrast, the increase in fluorescein clearance induced by I/R in oxygenated segments was only 264 ± 63% (P < 0.01 vs baseline). Intraluminal oxygenation afforded significant (P < 0.01) protection against I/R-induced mucosal hyperpermeability at all time points from T = 150-270 min. Histologic sections revealed complete villous epithelial denudation in nitrogenated segments, whereas the mucosal epithelium was largely intact in oxygenated segments. After reperfusion, mucosal ATP levels normalized in oxygenated segments, but there was only partial recovery of ATP levels in nitrogenated segments. These data indicate that intraluminal oxygenation ameliorates mucosal hyperpermeability induced by I/R.

AB - We tested the hypothesis that intraluminal oxygenation can prevent increased gut mucosal permeability to a hydrophilic solute caused by mesenteric ischemia/reperfusion (I/R). Studies were performed using pentobarbital-anesthetized immature pigs. Paired 20 cm segments of ileum were perfused intraluminally at 200 ml/hr with either nitrogenated or oxygenated 37°C Ringer's lactate. Beginning at T = -90 min, fluorescein disodium acetate was infused intravenously (12.5 mg/kg bolus, then 12.5 mg/kg/hr). Permeability was assessed by calculating the plasma-to-lumen clearance of fluorescein. Five pigs were subjected to complete mesenteric ischemia (T = 0- 120 min) followed by reperfusion (T = 120-270 min). In nitrogenated segments, I/R resulted in a 1015 ± 195% increase in fluorescein clearance (P < 0.01 vs baseline). In contrast, the increase in fluorescein clearance induced by I/R in oxygenated segments was only 264 ± 63% (P < 0.01 vs baseline). Intraluminal oxygenation afforded significant (P < 0.01) protection against I/R-induced mucosal hyperpermeability at all time points from T = 150-270 min. Histologic sections revealed complete villous epithelial denudation in nitrogenated segments, whereas the mucosal epithelium was largely intact in oxygenated segments. After reperfusion, mucosal ATP levels normalized in oxygenated segments, but there was only partial recovery of ATP levels in nitrogenated segments. These data indicate that intraluminal oxygenation ameliorates mucosal hyperpermeability induced by I/R.

KW - ATP

KW - fluorescein

KW - mucosa

KW - oxygen

KW - perfusion

KW - permeability

UR - http://www.scopus.com/inward/record.url?scp=0027231317&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027231317&partnerID=8YFLogxK

M3 - Article

C2 - 8324888

AN - SCOPUS:0027231317

VL - 40

SP - 37

EP - 46

JO - Journal of inflammation

JF - Journal of inflammation

SN - 1078-7852

IS - 1

ER -