Intermediates in the synthesis of TolC protein include an incomplete peptide stalled at a rare Arg codon

Rajeev MISRA, Peter REEVES

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

TolC is a minor outer membrane protein of Escherichia coli K 12 and is initially synthesized as a precursor. A distinct intermediate polypeptide of Mr about 46000 was consistently observed at the initial stages of biosynthesis. The further elongation of this peptide can be blocked by chloramphenicol. We have investigated the cause of the temporary accumulation of the 46000‐Mr intermediate and we postulate that the presence of a rare codon AGA (Arg) at codon 402 of the tolC mRNA halts translating ribosomes owing to a limiting amount of the tRNAArg (AGA) species in the cell. The translation of tolC mRNA can be increased by providing T4 tRNAArg(AGA), encoded on a plasmid.

Original languageEnglish (US)
Pages (from-to)151-155
Number of pages5
JournalEuropean Journal of Biochemistry
Volume152
Issue number1
DOIs
StatePublished - Oct 1985
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

Fingerprint Dive into the research topics of 'Intermediates in the synthesis of TolC protein include an incomplete peptide stalled at a rare Arg codon'. Together they form a unique fingerprint.

Cite this