Intermediates in the synthesis of TolC protein include an incomplete peptide stalled at a rare Arg codon

Rajeev Misra, P. Reeves

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

TolC is a minor outer membrane protein of Escherichia coli K12 and is initially synthesized as a precursor. A distinct intermediate polypeptide of M(r) about 46000 was consistently observed at the initial stages of biosynthesis. The further elongation of this peptide can be blocked by chloramphenicol. We have investigated the cause of the temporary accumulation of the 46000-M(r) intermediate and we postulate that the presence of a rare codon AGA (Arg) at codon 402 of the tolC mRNA halts translating ribosomes owing to a limiting amount of the tRNA(Arg) (AGA) species in the cell. The translation of tolC mRNA can be increased by providing T4 tRNA(Arg)(AGA), encoded on a plasmid.

Original languageEnglish (US)
Pages (from-to)151-155
Number of pages5
JournalEuropean Journal of Biochemistry
Volume152
Issue number1
StatePublished - 1985
Externally publishedYes

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RNA, Transfer, Arg
Codon
Escherichia coli K12
Messenger RNA
Peptides
Biosynthesis
Protein Biosynthesis
Chloramphenicol
Ribosomes
Escherichia coli
Elongation
Membrane Proteins
Proteins
Plasmids

ASJC Scopus subject areas

  • Biochemistry

Cite this

Intermediates in the synthesis of TolC protein include an incomplete peptide stalled at a rare Arg codon. / Misra, Rajeev; Reeves, P.

In: European Journal of Biochemistry, Vol. 152, No. 1, 1985, p. 151-155.

Research output: Contribution to journalArticle

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