Interleukin-1β stimulates mitogen-activated protein kinase in U373 astrocytoma cells without the production of lipid second messengers

A. K. Utal, A. L. Stopka, Paul Coleman

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

IL-1β is one of the cytokines known to affect astroglial cells in normal brain development, brain injury and neurodegenerative diseases. IL- 1β causes astrocytes to become more reactive, alter the expression and release of molecules and in some cases of proliferate. We have investigated the mitogenic effect and signal transduction pathway induced by IL-1β in U373 cells, a human astrocytoma cell-line. Recombinant human IL-1β induced mitogenesis of U373 cells in a dose-dependent fashion as assessed by tritiated thymidine incorporation. The following signal transduction mechanisms, reported to be induced in other systems by IL-1β, were investigated in U373 cells: (1) activation of phosphatidylcholine-specific phospholipase C as assayed by incorporation of tritiated choline into cellular phospholipids, (2) production of diacylglycerol, a lipid second messenger, (3) activation of sphingomyelinase, and (4) activation of mitogen- activated protein kinase (MAPK). Of these, IL-1β activated only MAPK. In cultured rat astrocytes, IL-1β caused activation of MAPK without inducing proliferation.

Original languageEnglish (US)
Pages (from-to)235-242
Number of pages8
JournalNeurochemical Research
Volume23
Issue number2
DOIs
StatePublished - Apr 2 1998
Externally publishedYes

Fingerprint

Astrocytoma
Second Messenger Systems
Mitogen-Activated Protein Kinases
Interleukin-1
Lipids
Chemical activation
Signal transduction
Astrocytes
Signal Transduction
Brain
Neurodegenerative diseases
Sphingomyelin Phosphodiesterase
Diglycerides
Brain Diseases
Choline
Neurodegenerative Diseases
Thymidine
Brain Injuries
Rats
Phospholipids

Keywords

  • Astrocytes
  • IL-1β
  • MAPK
  • Proliferation
  • Signal transduction

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Interleukin-1β stimulates mitogen-activated protein kinase in U373 astrocytoma cells without the production of lipid second messengers. / Utal, A. K.; Stopka, A. L.; Coleman, Paul.

In: Neurochemical Research, Vol. 23, No. 2, 02.04.1998, p. 235-242.

Research output: Contribution to journalArticle

@article{63a980511cab43819485289baab7606f,
title = "Interleukin-1β stimulates mitogen-activated protein kinase in U373 astrocytoma cells without the production of lipid second messengers",
abstract = "IL-1β is one of the cytokines known to affect astroglial cells in normal brain development, brain injury and neurodegenerative diseases. IL- 1β causes astrocytes to become more reactive, alter the expression and release of molecules and in some cases of proliferate. We have investigated the mitogenic effect and signal transduction pathway induced by IL-1β in U373 cells, a human astrocytoma cell-line. Recombinant human IL-1β induced mitogenesis of U373 cells in a dose-dependent fashion as assessed by tritiated thymidine incorporation. The following signal transduction mechanisms, reported to be induced in other systems by IL-1β, were investigated in U373 cells: (1) activation of phosphatidylcholine-specific phospholipase C as assayed by incorporation of tritiated choline into cellular phospholipids, (2) production of diacylglycerol, a lipid second messenger, (3) activation of sphingomyelinase, and (4) activation of mitogen- activated protein kinase (MAPK). Of these, IL-1β activated only MAPK. In cultured rat astrocytes, IL-1β caused activation of MAPK without inducing proliferation.",
keywords = "Astrocytes, IL-1β, MAPK, Proliferation, Signal transduction",
author = "Utal, {A. K.} and Stopka, {A. L.} and Paul Coleman",
year = "1998",
month = "4",
day = "2",
doi = "10.1023/A:1022493228015",
language = "English (US)",
volume = "23",
pages = "235--242",
journal = "Neurochemical Research",
issn = "0364-3190",
publisher = "Springer New York",
number = "2",

}

TY - JOUR

T1 - Interleukin-1β stimulates mitogen-activated protein kinase in U373 astrocytoma cells without the production of lipid second messengers

AU - Utal, A. K.

AU - Stopka, A. L.

AU - Coleman, Paul

PY - 1998/4/2

Y1 - 1998/4/2

N2 - IL-1β is one of the cytokines known to affect astroglial cells in normal brain development, brain injury and neurodegenerative diseases. IL- 1β causes astrocytes to become more reactive, alter the expression and release of molecules and in some cases of proliferate. We have investigated the mitogenic effect and signal transduction pathway induced by IL-1β in U373 cells, a human astrocytoma cell-line. Recombinant human IL-1β induced mitogenesis of U373 cells in a dose-dependent fashion as assessed by tritiated thymidine incorporation. The following signal transduction mechanisms, reported to be induced in other systems by IL-1β, were investigated in U373 cells: (1) activation of phosphatidylcholine-specific phospholipase C as assayed by incorporation of tritiated choline into cellular phospholipids, (2) production of diacylglycerol, a lipid second messenger, (3) activation of sphingomyelinase, and (4) activation of mitogen- activated protein kinase (MAPK). Of these, IL-1β activated only MAPK. In cultured rat astrocytes, IL-1β caused activation of MAPK without inducing proliferation.

AB - IL-1β is one of the cytokines known to affect astroglial cells in normal brain development, brain injury and neurodegenerative diseases. IL- 1β causes astrocytes to become more reactive, alter the expression and release of molecules and in some cases of proliferate. We have investigated the mitogenic effect and signal transduction pathway induced by IL-1β in U373 cells, a human astrocytoma cell-line. Recombinant human IL-1β induced mitogenesis of U373 cells in a dose-dependent fashion as assessed by tritiated thymidine incorporation. The following signal transduction mechanisms, reported to be induced in other systems by IL-1β, were investigated in U373 cells: (1) activation of phosphatidylcholine-specific phospholipase C as assayed by incorporation of tritiated choline into cellular phospholipids, (2) production of diacylglycerol, a lipid second messenger, (3) activation of sphingomyelinase, and (4) activation of mitogen- activated protein kinase (MAPK). Of these, IL-1β activated only MAPK. In cultured rat astrocytes, IL-1β caused activation of MAPK without inducing proliferation.

KW - Astrocytes

KW - IL-1β

KW - MAPK

KW - Proliferation

KW - Signal transduction

UR - http://www.scopus.com/inward/record.url?scp=0031912029&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031912029&partnerID=8YFLogxK

U2 - 10.1023/A:1022493228015

DO - 10.1023/A:1022493228015

M3 - Article

VL - 23

SP - 235

EP - 242

JO - Neurochemical Research

JF - Neurochemical Research

SN - 0364-3190

IS - 2

ER -