Intergenic variable-number tandem-repeat polymorphism upstream of rocA alters toxin production and enhances virulence in Streptococcus pyogenes

Luchang Zhu, Randall J. Olsen, Nicola Horstmann, Samuel A. Shelburne, Jia Fan, Ye Hu, James M. Musser

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Variable-number tandem-repeat (VNTR) polymorphisms are ubiquitous in bacteria. However, only a small fraction of them has been functionally studied. Here, we report an intergenic VNTR polymorphism that confers an altered level of toxin production and increased virulence in Streptococcus pyogenes. The nature of the polymorphism is a one-unit deletion in a three-tandemrepeat locus upstream of the rocA gene encoding a sensor kinase. S. pyogenes strains with this type of polymorphism cause human infection and produce significantly larger amounts of the secreted cytotoxins S. pyogenes NADase (SPN) and streptolysin O (SLO). Using isogenic mutant strains, we demonstrate that deleting one or more units of the tandem repeats abolished RocA production, reduced CovR phosphorylation, derepressed multiple CovR-regulated virulence factors (such as SPN and SLO), and increased virulence in a mouse model of necrotizing fasciitis. The phenotypic effect of the VNTR polymorphism was nearly the same as that of inactivating the rocA gene. In summary, we identified and characterized an intergenic VNTR polymorphism in S. pyogenes that affects toxin production and virulence. These new findings enhance understanding of rocA biology and the function of VNTR polymorphisms in S. pyogenes.

Original languageEnglish (US)
Pages (from-to)2086-2093
Number of pages8
JournalInfection and Immunity
Volume84
Issue number7
DOIs
StatePublished - Jan 1 2016
Externally publishedYes

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Minisatellite Repeats
Streptococcus pyogenes
Virulence
NAD+ Nucleosidase
Necrotizing Fasciitis
Tandem Repeat Sequences
Cytotoxins
Virulence Factors
Genes
Phosphotransferases
Phosphorylation
Bacteria
Infection

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Intergenic variable-number tandem-repeat polymorphism upstream of rocA alters toxin production and enhances virulence in Streptococcus pyogenes. / Zhu, Luchang; Olsen, Randall J.; Horstmann, Nicola; Shelburne, Samuel A.; Fan, Jia; Hu, Ye; Musser, James M.

In: Infection and Immunity, Vol. 84, No. 7, 01.01.2016, p. 2086-2093.

Research output: Contribution to journalArticle

Zhu, Luchang ; Olsen, Randall J. ; Horstmann, Nicola ; Shelburne, Samuel A. ; Fan, Jia ; Hu, Ye ; Musser, James M. / Intergenic variable-number tandem-repeat polymorphism upstream of rocA alters toxin production and enhances virulence in Streptococcus pyogenes. In: Infection and Immunity. 2016 ; Vol. 84, No. 7. pp. 2086-2093.
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