TY - JOUR
T1 - Interferometric plasmonic imaging and detection of single exosomes
AU - Yang, Yuting
AU - Shen, Guangxia
AU - Wang, Hui
AU - Li, Hongxia
AU - Zhang, Ting
AU - Tao, Nongjian
AU - Ding, Xianting
AU - Yu, Hui
N1 - Funding Information:
ACKNOWLEDGMENTS. The authors thank the Shanghai Pujiang Program (Grant 18PJ1406400), the National Natural Science Foundation of China (Grant 31771088), and the National Key Research and Development Program of China (Grants 2017YFC0107603 and 2017ZX10203205-006-002) for financial support.
Publisher Copyright:
© 2018 National Academy of Sciences. All rights reserved.
PY - 2018/10/9
Y1 - 2018/10/9
N2 - Exosomes play an important role in numerous cellular processes. Fundamental study and practical use of exosomes are significantly constrained by the lack of analytical tools capable of physical and biochemical characterization. In this paper, we present an optical approach capable of imaging single exosomes in a label-free manner, using interferometric plasmonic microscopy. We demonstrate monitoring of the real-time adsorption of exosomes onto a chemically modified Au surface, calculating the image intensity, and determining the size distribution. The sizing capability enables us to quantitatively measure the membrane fusion activity between exosomes and liposomes. We also report the recording of the dynamic interaction between exosomes and antibodies at the single-exosome level, and the tracking of hit-stay-run behavior of exosomes on an antibody-coated surface. We anticipate that the proposed method will contribute to clinical exosome analysis and to the exploration of fundamental issues such as the exosome- A ntibody binding kinetics.
AB - Exosomes play an important role in numerous cellular processes. Fundamental study and practical use of exosomes are significantly constrained by the lack of analytical tools capable of physical and biochemical characterization. In this paper, we present an optical approach capable of imaging single exosomes in a label-free manner, using interferometric plasmonic microscopy. We demonstrate monitoring of the real-time adsorption of exosomes onto a chemically modified Au surface, calculating the image intensity, and determining the size distribution. The sizing capability enables us to quantitatively measure the membrane fusion activity between exosomes and liposomes. We also report the recording of the dynamic interaction between exosomes and antibodies at the single-exosome level, and the tracking of hit-stay-run behavior of exosomes on an antibody-coated surface. We anticipate that the proposed method will contribute to clinical exosome analysis and to the exploration of fundamental issues such as the exosome- A ntibody binding kinetics.
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U2 - 10.1073/pnas.1804548115
DO - 10.1073/pnas.1804548115
M3 - Article
C2 - 30249664
AN - SCOPUS:85054772570
SN - 0027-8424
VL - 115
SP - 10275
EP - 10280
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 41
ER -