Interactions of TRF2 with model telomeric ends

Sheik J. Khan, Giscard Yanez, Kenneth Seldeen, Hongda Wang, Stuart Lindsay, Terace M. Fletcher

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Telomeres are DNA-protein complexes at the ends of eukaryotic chromosomes, the integrity of which is essential for chromosome stability. An important telomere binding protein, TTAGGG repeat factor 2 (TRF2), is thought to protect telomere ends by remodeling them into T-loops. We show that TRF2 specifically interacts with telomeric ss/ds DNA junctions and binding is sensitive to the sequence of the 3′, guanine-strand (G-strand) overhang and double-stranded DNA sequence at the junction. Association of TRF2 with DNA junctions hinders cleavage by exonuclease T. TRF2 interactions with the G-strand overhang do not involve the TRF2 DNA binding domain or the linker region. However, mobility shifts and atomic force microscopy show that the previously uncharacterized linker region is involved in DNA-specific, TRF2 oligomerization. We suggest that T-loop formation at telomere ends involves TRF2 binding to the G-strand overhang and oligomerization through both the known TRFH domain and the linker region.

Original languageEnglish (US)
Pages (from-to)44-50
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume363
Issue number1
DOIs
StatePublished - Nov 9 2007

Fingerprint

DNA
Telomere
Guanine
Oligomerization
Chromosomes
Telomere-Binding Proteins
Exonucleases
Chromosomal Instability
Atomic Force Microscopy
DNA sequences
Atomic force microscopy
Proteins

Keywords

  • DNA structure
  • G-quadruplex
  • Telomere
  • TRF2

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Interactions of TRF2 with model telomeric ends. / Khan, Sheik J.; Yanez, Giscard; Seldeen, Kenneth; Wang, Hongda; Lindsay, Stuart; Fletcher, Terace M.

In: Biochemical and Biophysical Research Communications, Vol. 363, No. 1, 09.11.2007, p. 44-50.

Research output: Contribution to journalArticle

Khan, Sheik J. ; Yanez, Giscard ; Seldeen, Kenneth ; Wang, Hongda ; Lindsay, Stuart ; Fletcher, Terace M. / Interactions of TRF2 with model telomeric ends. In: Biochemical and Biophysical Research Communications. 2007 ; Vol. 363, No. 1. pp. 44-50.
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