TY - JOUR
T1 - Interaction of positive and negative regulatory proteins in the galactose regulon of yeast
AU - Johnston, Stephen A.
AU - Salmeron, John M.
AU - Dincher, Sandra S.
N1 - Funding Information:
We thank K. Matsumoto and Y. Oshima for providing strains. This work was supported by National Science Foundation grant DCB-8502828 to S. A. J., and National Institutes of Health Genetics Training Grant 5T32-GM07754 to J. M. S. and S. S. D. We thank Glenda Winston for preparing the manuscript.
PY - 1987/7/3
Y1 - 1987/7/3
N2 - Transcriptional regulation in the galactose regulon of yeast is determined by an interplay between a positive regulatory protein, GAL4, and a negative regulatory protein, GAL80. We show that derivatives of GAL4 missing as few as 28 carboxy-terminal amino acids are not responsive to GAL80 regulation, implying that the carboxyl terminus of GAL4 is required for interaction with GAL80. Furthermore, a lesion in GAL4 that genetically defines the GAL80-interactive region maps to the 3′ end of the gene. Since the carboxyl terminus of GAL4 has also been implicated in interaction with a transcriptional factor, we propose a model for negative regulation in which GAL80 and this factor compete for binding a common region of GAL4, and the on/off state of transcription is determined by the relative affinities of the negative regulator and the transcriptional factor for this region.
AB - Transcriptional regulation in the galactose regulon of yeast is determined by an interplay between a positive regulatory protein, GAL4, and a negative regulatory protein, GAL80. We show that derivatives of GAL4 missing as few as 28 carboxy-terminal amino acids are not responsive to GAL80 regulation, implying that the carboxyl terminus of GAL4 is required for interaction with GAL80. Furthermore, a lesion in GAL4 that genetically defines the GAL80-interactive region maps to the 3′ end of the gene. Since the carboxyl terminus of GAL4 has also been implicated in interaction with a transcriptional factor, we propose a model for negative regulation in which GAL80 and this factor compete for binding a common region of GAL4, and the on/off state of transcription is determined by the relative affinities of the negative regulator and the transcriptional factor for this region.
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U2 - 10.1016/0092-8674(87)90671-4
DO - 10.1016/0092-8674(87)90671-4
M3 - Article
C2 - 3297350
AN - SCOPUS:0023643044
SN - 0092-8674
VL - 50
SP - 143
EP - 146
JO - Cell
JF - Cell
IS - 1
ER -