Interaction of graphene oxide with bacterial cell membranes

Insights from force spectroscopy

Santiago Romero-Vargas Castrillón, Francois Perreault, Andreia Fonseca De Faria, Menachem Elimelech

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

Understanding the interactions of graphene oxide (GO) with biological membranes is crucial for the evaluation of GO's health and environmental impacts, its bactericidal activity, and to advance graphene-based biological and environmental applications. In an effort to understand graphene-induced bacterial inactivation, we studied the interaction of GO with bacterial (Escherichia coli) cell membranes using atomic force microscopy (AFM). Toward this goal, we devised a polydopamine-assisted experimental protocol to functionalize an AFM probe with GO nanosheets, and used AFM-based force spectroscopy to measure cell membrane-GO interaction forces. Our results show that GO-cell interactions are predominantly repulsive, with only sporadic adhesion forces being measured upon probe pull-off, which we attribute to lipopolysaccharide bridging. We provide evidence of the acellular oxidation of glutathione by GO, underscoring the role of oxidative pathways in GO-mediated bacterial cell inactivation. Our force spectroscopy results suggest that physicochemical interactions do not underlie the primary mode of action of GO in bacteria.

Original languageEnglish (US)
Pages (from-to)112-117
Number of pages6
JournalEnvironmental Science and Technology Letters
Volume2
Issue number4
DOIs
StatePublished - Apr 14 2015
Externally publishedYes

Fingerprint

Graphite
Cell membranes
Oxides
Graphene
Spectrum Analysis
spectroscopy
Cell Membrane
oxide
Spectroscopy
membrane
atomic force microscopy
Atomic Force Microscopy
Atomic force microscopy
probe
health impact
Biological membranes
adhesion
Nanosheets
environmental impact
Cell Communication

ASJC Scopus subject areas

  • Ecology
  • Environmental Chemistry
  • Health, Toxicology and Mutagenesis
  • Pollution
  • Waste Management and Disposal
  • Water Science and Technology

Cite this

Interaction of graphene oxide with bacterial cell membranes : Insights from force spectroscopy. / Romero-Vargas Castrillón, Santiago; Perreault, Francois; De Faria, Andreia Fonseca; Elimelech, Menachem.

In: Environmental Science and Technology Letters, Vol. 2, No. 4, 14.04.2015, p. 112-117.

Research output: Contribution to journalArticle

Romero-Vargas Castrillón, Santiago ; Perreault, Francois ; De Faria, Andreia Fonseca ; Elimelech, Menachem. / Interaction of graphene oxide with bacterial cell membranes : Insights from force spectroscopy. In: Environmental Science and Technology Letters. 2015 ; Vol. 2, No. 4. pp. 112-117.
@article{08a0f541a7584748b81818e2ac662065,
title = "Interaction of graphene oxide with bacterial cell membranes: Insights from force spectroscopy",
abstract = "Understanding the interactions of graphene oxide (GO) with biological membranes is crucial for the evaluation of GO's health and environmental impacts, its bactericidal activity, and to advance graphene-based biological and environmental applications. In an effort to understand graphene-induced bacterial inactivation, we studied the interaction of GO with bacterial (Escherichia coli) cell membranes using atomic force microscopy (AFM). Toward this goal, we devised a polydopamine-assisted experimental protocol to functionalize an AFM probe with GO nanosheets, and used AFM-based force spectroscopy to measure cell membrane-GO interaction forces. Our results show that GO-cell interactions are predominantly repulsive, with only sporadic adhesion forces being measured upon probe pull-off, which we attribute to lipopolysaccharide bridging. We provide evidence of the acellular oxidation of glutathione by GO, underscoring the role of oxidative pathways in GO-mediated bacterial cell inactivation. Our force spectroscopy results suggest that physicochemical interactions do not underlie the primary mode of action of GO in bacteria.",
author = "{Romero-Vargas Castrill{\'o}n}, Santiago and Francois Perreault and {De Faria}, {Andreia Fonseca} and Menachem Elimelech",
year = "2015",
month = "4",
day = "14",
doi = "10.1021/acs.estlett.5b00066",
language = "English (US)",
volume = "2",
pages = "112--117",
journal = "Environmental Science and Technology Letters",
issn = "2328-8930",
publisher = "American Chemical Society",
number = "4",

}

TY - JOUR

T1 - Interaction of graphene oxide with bacterial cell membranes

T2 - Insights from force spectroscopy

AU - Romero-Vargas Castrillón, Santiago

AU - Perreault, Francois

AU - De Faria, Andreia Fonseca

AU - Elimelech, Menachem

PY - 2015/4/14

Y1 - 2015/4/14

N2 - Understanding the interactions of graphene oxide (GO) with biological membranes is crucial for the evaluation of GO's health and environmental impacts, its bactericidal activity, and to advance graphene-based biological and environmental applications. In an effort to understand graphene-induced bacterial inactivation, we studied the interaction of GO with bacterial (Escherichia coli) cell membranes using atomic force microscopy (AFM). Toward this goal, we devised a polydopamine-assisted experimental protocol to functionalize an AFM probe with GO nanosheets, and used AFM-based force spectroscopy to measure cell membrane-GO interaction forces. Our results show that GO-cell interactions are predominantly repulsive, with only sporadic adhesion forces being measured upon probe pull-off, which we attribute to lipopolysaccharide bridging. We provide evidence of the acellular oxidation of glutathione by GO, underscoring the role of oxidative pathways in GO-mediated bacterial cell inactivation. Our force spectroscopy results suggest that physicochemical interactions do not underlie the primary mode of action of GO in bacteria.

AB - Understanding the interactions of graphene oxide (GO) with biological membranes is crucial for the evaluation of GO's health and environmental impacts, its bactericidal activity, and to advance graphene-based biological and environmental applications. In an effort to understand graphene-induced bacterial inactivation, we studied the interaction of GO with bacterial (Escherichia coli) cell membranes using atomic force microscopy (AFM). Toward this goal, we devised a polydopamine-assisted experimental protocol to functionalize an AFM probe with GO nanosheets, and used AFM-based force spectroscopy to measure cell membrane-GO interaction forces. Our results show that GO-cell interactions are predominantly repulsive, with only sporadic adhesion forces being measured upon probe pull-off, which we attribute to lipopolysaccharide bridging. We provide evidence of the acellular oxidation of glutathione by GO, underscoring the role of oxidative pathways in GO-mediated bacterial cell inactivation. Our force spectroscopy results suggest that physicochemical interactions do not underlie the primary mode of action of GO in bacteria.

UR - http://www.scopus.com/inward/record.url?scp=84969219457&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84969219457&partnerID=8YFLogxK

U2 - 10.1021/acs.estlett.5b00066

DO - 10.1021/acs.estlett.5b00066

M3 - Article

VL - 2

SP - 112

EP - 117

JO - Environmental Science and Technology Letters

JF - Environmental Science and Technology Letters

SN - 2328-8930

IS - 4

ER -