Interaction between complementary polymerization sites in the structural D and E domains of human fibrin

Tatiana Ugarova, Andrei Z. Budzynski

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Abstract

Plasmic degradation products of human fibrin, fragments DD, D, and E, bind to fibrin. It has been inferred from this observation that the binding occurs by attraction of complementary sites located in the NH2-and COOH-terminal domains of the fibrin molecule. The interaction between fragments D1 and E1 has been investigated in this work since it represents the first step in the process of fibrin clot formation. Fragment D1, that was initially as active as fragment DD, lost most of its anticoagulant activity after purification by cation-exchange chromatography. The lability of fragment D1 function explained the previous unsuccessful attempts to form a complex between fragments D1 and E1. The loss of fragment D1 anticoagulant activity was not associated with the cleavage of the γ63-85 chain segment, since fragments D1A and D1 identically inhibited the fibrin monomer polymerization rate. In order to demonstrate the formation of a complex between fragments D1 and E1, three lines of experiments were advanced. First, the anticoagulant activity of fragment D1 was neutralized by fragment E1 in a dose-dependent manner, demonstrating that the association between these fragments involved polymerization sites. Second, two products, D1·E1 and D1·E1·D1, were stabilized in a reaction with bifunctional cross-linking reagents, proving the formation of D·E complexes in aqueous solution. Third, immobilized fragment D1 bound fragments E1 and E2, but not fragment E3, showing that fragments E1 and E2 attached via a polymerization site to the complementary one in fragment D1, since this association was disrupted by fibrin polymerization inhibitory peptide GPRP. These results provided direct evidence for specific binding between the structural D and E domains of fibrin mediated through complementary polymerization sites. Thus, the initial formation of fibrin clot fibers appears to be driven by specific association of these sites.

Original languageEnglish (US)
Pages (from-to)13687-13693
Number of pages7
JournalJournal of Biological Chemistry
Volume267
Issue number19
StatePublished - Jul 5 1992
Externally publishedYes

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Fibrin
Polymerization
Anticoagulants
Cross-Linking Reagents
Association reactions
glycyl-prolyl-arginyl-proline
Chromatography
Purification
Cations
Ion exchange
Degradation
Molecules
Fibers
Experiments

ASJC Scopus subject areas

  • Biochemistry

Cite this

Interaction between complementary polymerization sites in the structural D and E domains of human fibrin. / Ugarova, Tatiana; Budzynski, Andrei Z.

In: Journal of Biological Chemistry, Vol. 267, No. 19, 05.07.1992, p. 13687-13693.

Research output: Contribution to journalArticle

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