Integration of user interface, device control, data acquisition and analysis for automated multi-spectral imaging of single biological cells

Clement S. Sun, James R. Etzkorn, Mark R. Holl, Timothy W. Molter, Shawn K. Mcguire, Sarah C. McQuaide, Lloyd W. Burgess, Mary E. Lidstrom, Deirdre Meldrum

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

Major advances in the understanding in the biological sciences have been achieved by the precision automation of repetitive analytical procedures. The requirements for precision are pushed to the extreme when considering the analysis of cell function at the single cell level. Such analyses may include examination of gene expression, protein synthesis, and metabolic activity. The repetitive nature of performing measurement of these parameters requires not only effective signal transduction and amplification, significant attention to system integration and automation is paramount to instrument development objectives. Here we present the systems integration and software development for a multi-spectral imaging and phosphorescence lifetime measurement tool. This tool represents and early engineering prototype of one subsystem of an integrated automation pipeline comprising robotics for micro well-array movement, microwell-array sealing for metabolic rate measurements, control of experimental protocol sequencing and real-time imaging of dynamic experiments. A graphical user interface and underlying control software allows for flexible construction of experimental procedures. The engineering prototype has demonstrated experimental operation times up to 10 times faster than previous manual methods.

Original languageEnglish (US)
Title of host publication4th IEEE Conference on Automation Science and Engineering, CASE 2008
Pages1013-1018
Number of pages6
DOIs
StatePublished - Nov 3 2008
Event4th IEEE Conference on Automation Science and Engineering, CASE 2008 - Washington, DC, United States
Duration: Aug 23 2008Aug 26 2008

Publication series

Name4th IEEE Conference on Automation Science and Engineering, CASE 2008

Other

Other4th IEEE Conference on Automation Science and Engineering, CASE 2008
Country/TerritoryUnited States
CityWashington, DC
Period8/23/088/26/08

ASJC Scopus subject areas

  • Control and Systems Engineering
  • Electrical and Electronic Engineering

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