Integration of physical, breakpoint and genetic maps of chromosome 22. Localization of 587 yeast artificial chromosomes with 238 mapped markers

Callum J.bell, Marcia L.budarf, Bart W.nieuwenhuijsen, Barry L.barnoski, Kenneth H.buetow, Keely Campbell, Angela M.E.Colbert, Joelle Collins, Mark Daly, Philippe R.desjardins, Todd Dezwaan, Barbara Eckman, Simon Foote, Kyle Hart, Kevin Hiester, Marius J.van Het Hoog, Elizabeth Hopper, Alan Kaufman, Heather E.mcdermid, G. Christian OvertonMary Pat Reeve, David B.searls, Lincoln Stein, Vinay H.valmiki, Edward Watson, Sloan Williams, Rachel Winston, Robert L. Nussbaum, Eric S.lander, Kenneth H.fischbeck, Beverly S.emanuel, Thomas J.hudson

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

Detailed physical maps of the human genome are important resources for the Identification and isolation of disease genes and for studying the structure and function of the genome. We used data from STS content mapping of YACs and natural and induced chromosomal breakpoints to anchor contigs of overlapping yeast artificial chromosome (YAC) clones spanning extensive regions of human chromosome 22. The STSs were assigned to specific regions (bins) on the chromosome using cell lines from a somatic hybrid mapping panel defining a maximum of 25 intervals. YAC libraries were screened by PCR amplification of hierarchical pools of yeast DNA with 238 markers, and a total of 587 YAC clones were identified. These YACs were assembled into contigs based upon their shared STS content using a simulated annealing algorithm. Fifteen contigs, containing between 2 and 74 STSs were assembled, and ordered along the chromosome based upon the cytogenetic breakpoint, meiotic and PFG maps. Additional singleton YACs were assigned to unique chromosomal bins. These ordered YAC contigs will be useful for identifying disease genes and chromosomal breakpoints by positional cloning and will provide the foundation for higher resolution physical maps for large scale sequencing of the chromosome.

Original languageEnglish (US)
Pages (from-to)59-69
Number of pages11
JournalHuman Molecular Genetics
Volume4
Issue number1
DOIs
StatePublished - Jan 1 1995
Externally publishedYes

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ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

Cite this

J.bell, C., L.budarf, M., W.nieuwenhuijsen, B., L.barnoski, B., H.buetow, K., Campbell, K., M.E.Colbert, A., Collins, J., Daly, M., R.desjardins, P., Dezwaan, T., Eckman, B., Foote, S., Hart, K., Hiester, K., Hoog, M. J. V. H., Hopper, E., Kaufman, A., E.mcdermid, H., ... J.hudson, T. (1995). Integration of physical, breakpoint and genetic maps of chromosome 22. Localization of 587 yeast artificial chromosomes with 238 mapped markers. Human Molecular Genetics, 4(1), 59-69. https://doi.org/10.1093/hmg/4.1.59