TY - JOUR
T1 - Integration of physical, breakpoint and genetic maps of chromosome 22. Localization of 587 yeast artificial chromosomes with 238 mapped markers
AU - J.bell, Callum
AU - L.budarf, Marcia
AU - W.nieuwenhuijsen, Bart
AU - L.barnoski, Barry
AU - H.buetow, Kenneth
AU - Campbell, Keely
AU - M.E.Colbert, Angela
AU - Collins, Joelle
AU - Daly, Mark
AU - R.desjardins, Philippe
AU - Dezwaan, Todd
AU - Eckman, Barbara
AU - Foote, Simon
AU - Hart, Kyle
AU - Hiester, Kevin
AU - Hoog, Marius J.van Het
AU - Hopper, Elizabeth
AU - Kaufman, Alan
AU - E.mcdermid, Heather
AU - Overton, G. Christian
AU - Reeve, Mary Pat
AU - B.searls, David
AU - Stein, Lincoln
AU - H.valmiki, Vinay
AU - Watson, Edward
AU - Williams, Sloan
AU - Winston, Rachel
AU - Nussbaum, Robert L.
AU - S.lander, Eric
AU - H.fischbeck, Kenneth
AU - S.emanuel, Beverly
AU - J.hudson, Thomas
N1 - Funding Information:
The work undertaken in the Human Genome Center for Chromosome 22 in Philadelphia was supported by grant numbers P50-HG00425 (NCHGR) and CA39926 (NCI) from the NIH. Studies in the Whitehead Institute/MIT Center for Genome Research were supported by National Institute of Health Center for Genome Research Grant P50-HG00098. We wish to thank Eric Green and Glen Evans for screening for Washington University YACs, Eckart Meese and Marco Giovannini for providing STSs prior to publication, Daniel Cohen, Ilya Chumakov and Jean Weissenbach for the CEPH YAC libraries and the Alu-PCR generated chromosome 22 subset, and Willem Van Loon for biomek routines. Thomas Hudson is a recipient of a Clinician-Scientist Award from the Medical Research Council of Canada.
PY - 1995/1
Y1 - 1995/1
N2 - Detailed physical maps of the human genome are important resources for the Identification and isolation of disease genes and for studying the structure and function of the genome. We used data from STS content mapping of YACs and natural and induced chromosomal breakpoints to anchor contigs of overlapping yeast artificial chromosome (YAC) clones spanning extensive regions of human chromosome 22. The STSs were assigned to specific regions (bins) on the chromosome using cell lines from a somatic hybrid mapping panel defining a maximum of 25 intervals. YAC libraries were screened by PCR amplification of hierarchical pools of yeast DNA with 238 markers, and a total of 587 YAC clones were identified. These YACs were assembled into contigs based upon their shared STS content using a simulated annealing algorithm. Fifteen contigs, containing between 2 and 74 STSs were assembled, and ordered along the chromosome based upon the cytogenetic breakpoint, meiotic and PFG maps. Additional singleton YACs were assigned to unique chromosomal bins. These ordered YAC contigs will be useful for identifying disease genes and chromosomal breakpoints by positional cloning and will provide the foundation for higher resolution physical maps for large scale sequencing of the chromosome.
AB - Detailed physical maps of the human genome are important resources for the Identification and isolation of disease genes and for studying the structure and function of the genome. We used data from STS content mapping of YACs and natural and induced chromosomal breakpoints to anchor contigs of overlapping yeast artificial chromosome (YAC) clones spanning extensive regions of human chromosome 22. The STSs were assigned to specific regions (bins) on the chromosome using cell lines from a somatic hybrid mapping panel defining a maximum of 25 intervals. YAC libraries were screened by PCR amplification of hierarchical pools of yeast DNA with 238 markers, and a total of 587 YAC clones were identified. These YACs were assembled into contigs based upon their shared STS content using a simulated annealing algorithm. Fifteen contigs, containing between 2 and 74 STSs were assembled, and ordered along the chromosome based upon the cytogenetic breakpoint, meiotic and PFG maps. Additional singleton YACs were assigned to unique chromosomal bins. These ordered YAC contigs will be useful for identifying disease genes and chromosomal breakpoints by positional cloning and will provide the foundation for higher resolution physical maps for large scale sequencing of the chromosome.
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U2 - 10.1093/hmg/4.1.59
DO - 10.1093/hmg/4.1.59
M3 - Article
C2 - 7711735
AN - SCOPUS:0028814128
SN - 0964-6906
VL - 4
SP - 59
EP - 69
JO - Human Molecular Genetics
JF - Human Molecular Genetics
IS - 1
ER -