Integration of a foreign gene into a native complex does not impair fitness in an experimental model of lateral gene transfer

Shira Omer, Amir Kovacs, Yuval Mazor, Uri Gophna

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Lateral gene transfer (LGT) is a central force in microbial evolution. The observation that genes encoding subunits of complexes exhibit relatively compatible phylogenies, suggesting vertical descent, can be explained by different evolutionary scenarios. On the one hand, the failure of a new gene product to correctly interact with preexisting protein subunits can make its acquisition neutral-a theory termed the "complexity hypothesis." On the other hand, foreign subunit-encoding genes may reduce the fitness of the new host by disrupting the stoichiometric balance between complex subunits, resulting in purifying selection against gene retention. We previously showed in a model LGT system that overexpression of an orthologous subunit was neutral due to lack of interaction with host subunits. Here, we examine a case where the foreign protein is more similar to its native orthologs, by expressing the RNA polymerase β subunit (RpoB) of Bacillus subtilis in Escherichia coli. The foreign subunit is shown by coimmunoprecipitation to interact with the host subunits, and to form novel, nonspecific interactions. Nevertheless, the host did not incur any fitness disadvantage, as measured by its growth. We conclude that LGT of complex subunits may be neutral even when the transferred subunit can integrate into the host complex and that this neutrality can be a fertile ground for selective forces once the environment changes.

Original languageEnglish (US)
Pages (from-to)2441-2445
Number of pages5
JournalMolecular Biology and Evolution
Volume27
Issue number11
DOIs
StatePublished - Nov 2010
Externally publishedYes

Fingerprint

Horizontal Gene Transfer
gene transfer
fitness
Theoretical Models
gene
Genes
genes
Protein Subunits
DNA-Directed RNA Polymerases
Phylogeny
Bacillus subtilis
protein
protein subunits
DNA-directed RNA polymerase
RNA
Escherichia coli
phylogeny
horizontal gene transfer
Growth
Proteins

Keywords

  • balance hypothesis
  • complexity hypothesis
  • essential genes
  • horizontal gene transfer
  • protein complexes

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Molecular Biology

Cite this

Integration of a foreign gene into a native complex does not impair fitness in an experimental model of lateral gene transfer. / Omer, Shira; Kovacs, Amir; Mazor, Yuval; Gophna, Uri.

In: Molecular Biology and Evolution, Vol. 27, No. 11, 11.2010, p. 2441-2445.

Research output: Contribution to journalArticle

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