Integrating DNA strand-displacement circuitry with DNA tile self-assembly

David Yu Zhang; Rizal F. Hariadi; Harry M.T. Choi; Erik Winfree

doi:10.1038/ncomms2965

Integrating DNA strand-displacement circuitry with DNA tile self-assembly

David Yu Zhang, Rizal F. Hariadi, Harry M.T. Choi, Erik Winfree

Research output: Contribution to journal › Article › peer-review

150 Scopus citations

Abstract

DNA nanotechnology has emerged as a reliable and programmable way of controlling matter at the nanoscale through the specificity of Watson-Crick base pairing, allowing both complex self-assembled structures with nanometer precision and complex reaction networks implementing digital and analog behaviors. Here we show how two well-developed frameworks, DNA tile self-assembly and DNA strand-displacement circuits, can be systematically integrated to provide programmable kinetic control of self-assembly. We demonstrate the triggered and catalytic isothermal self-assembly of DNA nanotubes over 10 μm long from precursor DNA double-crossover tiles activated by an upstream DNA catalyst network. Integrating more sophisticated control circuits and tile systems could enable precise spatial and temporal organization of dynamic molecular structures.

Original language	English (US)
Article number	1965
Journal	Nature communications
Volume	4
DOIs	https://doi.org/10.1038/ncomms2965
State	Published - 2013
Externally published	Yes

ASJC Scopus subject areas

Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)

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10.1038/ncomms2965

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title = "Integrating DNA strand-displacement circuitry with DNA tile self-assembly",

abstract = "DNA nanotechnology has emerged as a reliable and programmable way of controlling matter at the nanoscale through the specificity of Watson-Crick base pairing, allowing both complex self-assembled structures with nanometer precision and complex reaction networks implementing digital and analog behaviors. Here we show how two well-developed frameworks, DNA tile self-assembly and DNA strand-displacement circuits, can be systematically integrated to provide programmable kinetic control of self-assembly. We demonstrate the triggered and catalytic isothermal self-assembly of DNA nanotubes over 10 μm long from precursor DNA double-crossover tiles activated by an upstream DNA catalyst network. Integrating more sophisticated control circuits and tile systems could enable precise spatial and temporal organization of dynamic molecular structures.",

author = "Zhang, {David Yu} and Hariadi, {Rizal F.} and Choi, {Harry M.T.} and Erik Winfree",

note = "Funding Information: This work was supported in part by National Science Foundations grants 0832824, 0829805, and 0728703 to E.W. This work was supported in part by a Hertz Foundation graduate fellowship, a Howard Hughes Medical Institute postdoctoral fellowship as part of the Life Sciences Research Foundation program, and NIH Award 1K99EB015331 to D.Y.Z.",

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AU - Hariadi, Rizal F.

AU - Choi, Harry M.T.

AU - Winfree, Erik

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PY - 2013

Y1 - 2013

N2 - DNA nanotechnology has emerged as a reliable and programmable way of controlling matter at the nanoscale through the specificity of Watson-Crick base pairing, allowing both complex self-assembled structures with nanometer precision and complex reaction networks implementing digital and analog behaviors. Here we show how two well-developed frameworks, DNA tile self-assembly and DNA strand-displacement circuits, can be systematically integrated to provide programmable kinetic control of self-assembly. We demonstrate the triggered and catalytic isothermal self-assembly of DNA nanotubes over 10 μm long from precursor DNA double-crossover tiles activated by an upstream DNA catalyst network. Integrating more sophisticated control circuits and tile systems could enable precise spatial and temporal organization of dynamic molecular structures.

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Integrating DNA strand-displacement circuitry with DNA tile self-assembly

Abstract

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