Abstract

Devices with 150μm diameter apertures were microfabricated in a silicon substrate using well-known cleanroom technologies for the measurement of ion channel proteins. The capacitance of the device was reduced with a 75μm SU-8 layer and the surface was made hydrophobic with chemically vapor deposited polytetrafluoroethylene. A novel approach using phase sensitive detection was then used to measure single channels of the bacterial transmembrane porin protein OmpF inserted into phospholipid bilayers formed across the aperture of the fabricated device.

Original languageEnglish (US)
Title of host publication2006 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2006 Technical Proceedings
Pages178-181
Number of pages4
StatePublished - Dec 8 2006
Event2006 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2006 Technical Proceedings - Boston, MA, United States
Duration: May 7 2006May 11 2006

Publication series

Name2006 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2006 Technical Proceedings
Volume2

Other

Other2006 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2006 Technical Proceedings
CountryUnited States
CityBoston, MA
Period5/7/065/11/06

Keywords

  • Ion channel
  • Lipid bilayers
  • Microfabrication
  • Sensor

ASJC Scopus subject areas

  • Engineering(all)

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  • Cite this

    Wilk, S. J., Petrossian, L., Goryll, M., Thornton, T., Goodnick, S., Tang, J. M., & Eisenberg, R. S. (2006). Integrated techniques for transmembrance protein sensing. In 2006 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2006 Technical Proceedings (pp. 178-181). (2006 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2006 Technical Proceedings; Vol. 2).