Abstract

Conductance measurement of single ion channels and related stochastic signals is a promising technique for the development of a functional biosensor. We present results showing that silicon substrates can be used as a low noise, universal platform for recording the electrical activity of single ion channels inserted into bilayer membranes. Bilayers were suspended on polytetrafluoroethylene coated, 150μm apertures etched into silicon substrates and then oxidized with common silicon processing techniques. The overall noise of the system was reduced with a 75μm thick SU-8 layer and integrated silver/silver chloride electrodes. Phase sensitive detection of single OmpF porin ion channels using the silicon device and a lock-in amplifier were made and demonstrate the system's capability as a sensor in high noise environments.

Original languageEnglish (US)
Title of host publicationProceedings of the Fourth IEEE Conference on Sensors 2005
Pages1165-1168
Number of pages4
DOIs
StatePublished - Dec 1 2005
EventFourth IEEE Conference on Sensors 2005 - Irvine, CA, United States
Duration: Oct 31 2005Nov 3 2005

Publication series

NameProceedings of IEEE Sensors
Volume2005

Other

OtherFourth IEEE Conference on Sensors 2005
CountryUnited States
CityIrvine, CA
Period10/31/0511/3/05

ASJC Scopus subject areas

  • Electrical and Electronic Engineering

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  • Cite this

    Wilk, S. J., Petrossian, L., Goryll, M., Thornton, T., Goodnick, S., Tang, J. M., & Eisenberg, R. S. (2005). Integrated platform for ion channel sensing. In Proceedings of the Fourth IEEE Conference on Sensors 2005 (pp. 1165-1168). [1597912] (Proceedings of IEEE Sensors; Vol. 2005). https://doi.org/10.1109/ICSENS.2005.1597912