Abstract

Conductance measurement of single ion channels and related stochastic signals is a promising technique for the development of a functional biosensor. We present results showing that silicon substrates can be used as a low noise, universal platform for recording the electrical activity of single ion channels inserted into bilayer membranes. Bilayers were suspended on polytetrafluoroethylene coated, 150μm apertures etched into silicon substrates and then oxidized with common silicon processing techniques. The overall noise of the system was reduced with a 75μm thick SU-8 layer and integrated silver/silver chloride electrodes. Phase sensitive detection of single OmpF porin ion channels using the silicon device and a lock-in amplifier were made and demonstrate the system's capability as a sensor in high noise environments.

Original languageEnglish (US)
Title of host publicationProceedings of IEEE Sensors
Pages1165-1168
Number of pages4
Volume2005
DOIs
StatePublished - 2005
EventFourth IEEE Conference on Sensors 2005 - Irvine, CA, United States
Duration: Oct 31 2005Nov 3 2005

Other

OtherFourth IEEE Conference on Sensors 2005
CountryUnited States
CityIrvine, CA
Period10/31/0511/3/05

Fingerprint

Silicon
Ions
Silver
Substrates
Polytetrafluoroethylenes
Biosensors
Membranes
Electrodes
Sensors
Processing

ASJC Scopus subject areas

  • Engineering (miscellaneous)
  • Electrical and Electronic Engineering

Cite this

Wilk, S. J., Petrossian, L., Goryll, M., Thornton, T., Goodnick, S., Tang, J. M., & Eisenberg, R. S. (2005). Integrated platform for ion channel sensing. In Proceedings of IEEE Sensors (Vol. 2005, pp. 1165-1168). [1597912] https://doi.org/10.1109/ICSENS.2005.1597912

Integrated platform for ion channel sensing. / Wilk, S. J.; Petrossian, L.; Goryll, Michael; Thornton, Trevor; Goodnick, Stephen; Tang, J. M.; Eisenberg, R. S.

Proceedings of IEEE Sensors. Vol. 2005 2005. p. 1165-1168 1597912.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Wilk, SJ, Petrossian, L, Goryll, M, Thornton, T, Goodnick, S, Tang, JM & Eisenberg, RS 2005, Integrated platform for ion channel sensing. in Proceedings of IEEE Sensors. vol. 2005, 1597912, pp. 1165-1168, Fourth IEEE Conference on Sensors 2005, Irvine, CA, United States, 10/31/05. https://doi.org/10.1109/ICSENS.2005.1597912
Wilk SJ, Petrossian L, Goryll M, Thornton T, Goodnick S, Tang JM et al. Integrated platform for ion channel sensing. In Proceedings of IEEE Sensors. Vol. 2005. 2005. p. 1165-1168. 1597912 https://doi.org/10.1109/ICSENS.2005.1597912
Wilk, S. J. ; Petrossian, L. ; Goryll, Michael ; Thornton, Trevor ; Goodnick, Stephen ; Tang, J. M. ; Eisenberg, R. S. / Integrated platform for ion channel sensing. Proceedings of IEEE Sensors. Vol. 2005 2005. pp. 1165-1168
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