Integrated PDMS/CMOS microsystem for autonomous incubation and imaging in cell culture studies

Jennifer M. Blain Christen, Andreas G. Andreou

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations

Abstract

We discuss the design, fabrication and testing of a hybrid microsystem for stand-alone cell culture, incubation and imaging. The micro-incubator is engineered through the integration of two CMOS dies. The first for the heater and temperature sensor, and a second die for imaging. A multilayer PDMS (polydimethylsiloxane) structures is used to create not only the fluidic structures but to encapsulate the CMOS dies. The microfluidic features include fluidic channels, a 4mm diameter, 30μL, culture well and a 25 micron thick PDMS membrane that covers the top of the culture well, acting as barrier to contaminants while allowing the cells to exchange gases with the ambient environment. The micro-incubator interface includes with a flexible polyimide ribbon cable and four fluidic ports. The complete structure has a size of (2.5×2.5×0.6cm3). We have employed the device to successfully culture BHK-21 cells autonomously over a sixty hour period in ambient environment.

Original languageEnglish (US)
Title of host publication2006 IEEE/NLM Life Science Systems and Applications Workshop, LiSA 2006
DOIs
StatePublished - Dec 1 2006
Externally publishedYes
Event2006 IEEE/NLM Life Science Systems and Applications Workshop, LiSA 2006 - Bethesda, MD, United States
Duration: Jul 13 2006Jul 14 2006

Publication series

Name2006 IEEE/NLM Life Science Systems and Applications Workshop, LiSA 2006

Other

Other2006 IEEE/NLM Life Science Systems and Applications Workshop, LiSA 2006
Country/TerritoryUnited States
CityBethesda, MD
Period7/13/067/14/06

ASJC Scopus subject areas

  • Health(social science)
  • Assessment and Diagnosis
  • Medicine(all)
  • Health Information Management
  • Electrical and Electronic Engineering
  • Human-Computer Interaction
  • Computer Science Applications
  • Signal Processing

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