Integrated label-free protein detection and separation in real time using confined surface plasmon resonance imaging

Nguyen Ly, Kyle Foley, Nongjian Tao

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

We demonstrate a label-free protein detection and separation technology for real-time monitoring of proteins in micro/nanofluidic channels, confined surface plasmon resonance imaging (confined-SPRi). This was achieved by fabricating ultrathin fluidic channels (500 nm high, 500 μm wide) directly on top of a specialized SPRi sensor surface. In this way, SPRi is uniquely used to detect proteins deep into the fluidic channel while maintaining high lateral accuracy of separated products. The channel fluid and proteins were driven electrokinetically under an external electric field. For this to occur, the metallic SPR sensor (46 nm of Au on 2 nm of Cr) was segmented into an array of squares (each 200 μm × 200 μm in size and spaced 8 μm apart) and coated with 30 nm of CYTOP polymer. In this work, we track label-free protein separation in real time through a simple cross-junction fluidic device with an 8-mm separation channel length under 30 V/cm electric field strength.

Original languageEnglish (US)
Pages (from-to)2546-2551
Number of pages6
JournalAnalytical Chemistry
Volume79
Issue number6
DOIs
StatePublished - Mar 15 2007

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Surface plasmon resonance
Labels
Imaging techniques
Fluidics
Proteins
Electric fields
Fluidic devices
Nanofluidics
Sensors
Polymers
Fluids
Monitoring

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Integrated label-free protein detection and separation in real time using confined surface plasmon resonance imaging. / Ly, Nguyen; Foley, Kyle; Tao, Nongjian.

In: Analytical Chemistry, Vol. 79, No. 6, 15.03.2007, p. 2546-2551.

Research output: Contribution to journalArticle

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