Integrated electrodes on a silicon based ion channel measurement platform

S. J. Wilk; L. Petrossian; Michael Goryll; Trevor Thornton; Stephen Goodnick; J. M. Tang; R. S. Eisenberg

doi:10.1016/j.bios.2007.03.030

Integrated electrodes on a silicon based ion channel measurement platform

S. J. Wilk, L. Petrossian, Michael Goryll, Trevor Thornton, Stephen Goodnick, J. M. Tang, R. S. Eisenberg

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

We demonstrate the microfabrication of a low-noise silicon based device with integrated silver/silver chloride electrodes used for the measurement of single ion channel proteins. An aperture of 150 μm diameter was etched in a silicon substrate using a deep silicon reactive ion etcher and passivated with 30 nm of polytetrafluoroethylene via chemical vapor deposition. The average recorded noise in measurements of lipid bilayers was reduced by a factor of four through patterning of a 75 μm thick SU-8 layer around the aperture. Integrated electrodes were fabricated on both sides of the device and used for repeatable, stable, giga-seal bilayer formations as well as characteristic measurements of the transmembrane protein OmpF porin.

Original language	English (US)
Pages (from-to)	183-190
Number of pages	8
Journal	Biosensors and Bioelectronics
Volume	23
Issue number	2
DOIs	https://doi.org/10.1016/j.bios.2007.03.030
State	Published - Sep 30 2007

Keywords

Ion channels
Lipid bilayers
Microfabrication

ASJC Scopus subject areas

Biotechnology
Biophysics
Biomedical Engineering
Electrochemistry

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10.1016/j.bios.2007.03.030

Cite this

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title = "Integrated electrodes on a silicon based ion channel measurement platform",

abstract = "We demonstrate the microfabrication of a low-noise silicon based device with integrated silver/silver chloride electrodes used for the measurement of single ion channel proteins. An aperture of 150 μm diameter was etched in a silicon substrate using a deep silicon reactive ion etcher and passivated with 30 nm of polytetrafluoroethylene via chemical vapor deposition. The average recorded noise in measurements of lipid bilayers was reduced by a factor of four through patterning of a 75 μm thick SU-8 layer around the aperture. Integrated electrodes were fabricated on both sides of the device and used for repeatable, stable, giga-seal bilayer formations as well as characteristic measurements of the transmembrane protein OmpF porin.",

keywords = "Ion channels, Lipid bilayers, Microfabrication",

author = "Wilk, {S. J.} and L. Petrossian and Michael Goryll and Trevor Thornton and Stephen Goodnick and Tang, {J. M.} and Eisenberg, {R. S.}",

note = "Funding Information: This work was supported by the Defense Advanced Research Projects Agency as part of the MOLDICE program. Student support in part was from the National Science Foundation IGERT program.",

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doi = "10.1016/j.bios.2007.03.030",

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T1 - Integrated electrodes on a silicon based ion channel measurement platform

AU - Wilk, S. J.

AU - Petrossian, L.

AU - Goryll, Michael

AU - Thornton, Trevor

AU - Goodnick, Stephen

AU - Tang, J. M.

AU - Eisenberg, R. S.

N1 - Funding Information: This work was supported by the Defense Advanced Research Projects Agency as part of the MOLDICE program. Student support in part was from the National Science Foundation IGERT program.

PY - 2007/9/30

Y1 - 2007/9/30

N2 - We demonstrate the microfabrication of a low-noise silicon based device with integrated silver/silver chloride electrodes used for the measurement of single ion channel proteins. An aperture of 150 μm diameter was etched in a silicon substrate using a deep silicon reactive ion etcher and passivated with 30 nm of polytetrafluoroethylene via chemical vapor deposition. The average recorded noise in measurements of lipid bilayers was reduced by a factor of four through patterning of a 75 μm thick SU-8 layer around the aperture. Integrated electrodes were fabricated on both sides of the device and used for repeatable, stable, giga-seal bilayer formations as well as characteristic measurements of the transmembrane protein OmpF porin.

AB - We demonstrate the microfabrication of a low-noise silicon based device with integrated silver/silver chloride electrodes used for the measurement of single ion channel proteins. An aperture of 150 μm diameter was etched in a silicon substrate using a deep silicon reactive ion etcher and passivated with 30 nm of polytetrafluoroethylene via chemical vapor deposition. The average recorded noise in measurements of lipid bilayers was reduced by a factor of four through patterning of a 75 μm thick SU-8 layer around the aperture. Integrated electrodes were fabricated on both sides of the device and used for repeatable, stable, giga-seal bilayer formations as well as characteristic measurements of the transmembrane protein OmpF porin.

KW - Ion channels

KW - Lipid bilayers

KW - Microfabrication

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JO - Biosensors and Bioelectronics

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Integrated electrodes on a silicon based ion channel measurement platform

Abstract

Keywords

ASJC Scopus subject areas

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