Insulin receptor substrate influences female caste development in honeybees

Florian Wolschin, Navdeep S. Mutti, Gro Amdam

Research output: Contribution to journalArticle

55 Scopus citations

Abstract

The insulin/insulin-like signalling (IIS) network is conserved among animals and is central to growth and development. In eusocial honeybees (Apis mellifera), IIS is hypothesized to shape female caste fate. We tested this hypothesis via RNA interference (RNAi) knockdown of the insulin receptor substrate (IRS) homologue, a key adaptor protein in IIS. Female larvae naturally develop into queens (reproductives) or workers (helpers) after being fed rich versus limited diets, respectively. Feeding larvae a rich diet mixed with dsRNA (double stranded RNA) targeting irs gene transcript decreased irs mRNA abundance and caused development of worker morphology. Controls receiving rich larval diet and control dsRNA developed queen morphology. Whole-body mass spectrometry profiling of larvae collected 72, 96 and 120 h after dsRNA treatments revealed proteomic differences between irs gene knockdowns and controls, including levels of hexamerin 110, a storage protein connected to natural caste differences. This journal is

Original languageEnglish (US)
Pages (from-to)112-115
Number of pages4
JournalBiology letters
Volume7
Issue number1
DOIs
StatePublished - Feb 23 2011

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Keywords

  • Apis mellifera
  • Insulin signalling
  • Proteomics
  • Queen bee
  • RNA interference
  • Worker bee

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Agricultural and Biological Sciences(all)

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