Insulin-like peptide genes in honey bee fat body respond differently to manipulation of social behavioral physiology

Kari Anne Nilsen, Kate E. Ihle, Katy Frederick, M. Kim Fondrk, Bente Smedal, Klaus Hartfelder, Gro Amdam

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Nutrient sensitive insulin-like peptides (ILPs) have profound effects on invertebrate metabolism, nutrient storage, fertility and aging. Many insects transcribe ILPs in specialized neurosecretory cells at changing levels correlated with life history. However, the major site of insect metabolism and nutrient storage is not the brain, but rather the fat body, where functions of ILP expression are rarely studied and poorly understood. Fat body is analogous to mammalian liver and adipose tissue, with nutrient stores that often correlate with behavior. We used the honey bee (Apis mellifera), an insect with complex behavior, to test whether ILP genes in fat body respond to experimentally induced changes of behavioral physiology. Honey bee fat body influences endocrine state and behavior by secreting the yolk protein precursor vitellogenin (Vg), which suppresses lipophilic juvenile hormone and social foraging behavior. In a two-factorial experiment, we used RNA interference (RNAi)-mediated vg gene knockdown and amino acid nutrient enrichment of hemolymph (blood) to perturb this regulatory module. We document factorspecific changes in fat body ilp1 and ilp2 mRNA, the bee's ILP-encoding genes, and confirm that our protocol affects social behavior. We show that ilp1 and ilp2 are regulated independently and differently and diverge in their specific expressionlocalization between fat body oenocyte and trophocyte cells. Insect ilp functions may be better understood by broadening research to account for expression in fat body and not only brain.

Original languageEnglish (US)
Pages (from-to)1488-1497
Number of pages10
JournalJournal of Experimental Biology
Volume214
Issue number9
DOIs
StatePublished - May 2011

Fingerprint

Behavior Control
Fat Body
Honey
Bees
honey
fat body
bee
peptide
fat
honey bees
physiology
insulin
peptides
Insulin
Peptides
gene
Insects
Food
Genes
insects

Keywords

  • Division of labor
  • Nutrition
  • Peripheral insulin-like signaling
  • RNA-interference
  • Vitellogenin

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Physiology
  • Insect Science
  • Aquatic Science

Cite this

Insulin-like peptide genes in honey bee fat body respond differently to manipulation of social behavioral physiology. / Nilsen, Kari Anne; Ihle, Kate E.; Frederick, Katy; Fondrk, M. Kim; Smedal, Bente; Hartfelder, Klaus; Amdam, Gro.

In: Journal of Experimental Biology, Vol. 214, No. 9, 05.2011, p. 1488-1497.

Research output: Contribution to journalArticle

Nilsen, Kari Anne ; Ihle, Kate E. ; Frederick, Katy ; Fondrk, M. Kim ; Smedal, Bente ; Hartfelder, Klaus ; Amdam, Gro. / Insulin-like peptide genes in honey bee fat body respond differently to manipulation of social behavioral physiology. In: Journal of Experimental Biology. 2011 ; Vol. 214, No. 9. pp. 1488-1497.
@article{5ea082e5e2644aca84fa5dda38077ae6,
title = "Insulin-like peptide genes in honey bee fat body respond differently to manipulation of social behavioral physiology",
abstract = "Nutrient sensitive insulin-like peptides (ILPs) have profound effects on invertebrate metabolism, nutrient storage, fertility and aging. Many insects transcribe ILPs in specialized neurosecretory cells at changing levels correlated with life history. However, the major site of insect metabolism and nutrient storage is not the brain, but rather the fat body, where functions of ILP expression are rarely studied and poorly understood. Fat body is analogous to mammalian liver and adipose tissue, with nutrient stores that often correlate with behavior. We used the honey bee (Apis mellifera), an insect with complex behavior, to test whether ILP genes in fat body respond to experimentally induced changes of behavioral physiology. Honey bee fat body influences endocrine state and behavior by secreting the yolk protein precursor vitellogenin (Vg), which suppresses lipophilic juvenile hormone and social foraging behavior. In a two-factorial experiment, we used RNA interference (RNAi)-mediated vg gene knockdown and amino acid nutrient enrichment of hemolymph (blood) to perturb this regulatory module. We document factorspecific changes in fat body ilp1 and ilp2 mRNA, the bee's ILP-encoding genes, and confirm that our protocol affects social behavior. We show that ilp1 and ilp2 are regulated independently and differently and diverge in their specific expressionlocalization between fat body oenocyte and trophocyte cells. Insect ilp functions may be better understood by broadening research to account for expression in fat body and not only brain.",
keywords = "Division of labor, Nutrition, Peripheral insulin-like signaling, RNA-interference, Vitellogenin",
author = "Nilsen, {Kari Anne} and Ihle, {Kate E.} and Katy Frederick and Fondrk, {M. Kim} and Bente Smedal and Klaus Hartfelder and Gro Amdam",
year = "2011",
month = "5",
doi = "10.1242/jeb.050393",
language = "English (US)",
volume = "214",
pages = "1488--1497",
journal = "Journal of Experimental Biology",
issn = "0022-0949",
publisher = "Company of Biologists Ltd",
number = "9",

}

TY - JOUR

T1 - Insulin-like peptide genes in honey bee fat body respond differently to manipulation of social behavioral physiology

AU - Nilsen, Kari Anne

AU - Ihle, Kate E.

AU - Frederick, Katy

AU - Fondrk, M. Kim

AU - Smedal, Bente

AU - Hartfelder, Klaus

AU - Amdam, Gro

PY - 2011/5

Y1 - 2011/5

N2 - Nutrient sensitive insulin-like peptides (ILPs) have profound effects on invertebrate metabolism, nutrient storage, fertility and aging. Many insects transcribe ILPs in specialized neurosecretory cells at changing levels correlated with life history. However, the major site of insect metabolism and nutrient storage is not the brain, but rather the fat body, where functions of ILP expression are rarely studied and poorly understood. Fat body is analogous to mammalian liver and adipose tissue, with nutrient stores that often correlate with behavior. We used the honey bee (Apis mellifera), an insect with complex behavior, to test whether ILP genes in fat body respond to experimentally induced changes of behavioral physiology. Honey bee fat body influences endocrine state and behavior by secreting the yolk protein precursor vitellogenin (Vg), which suppresses lipophilic juvenile hormone and social foraging behavior. In a two-factorial experiment, we used RNA interference (RNAi)-mediated vg gene knockdown and amino acid nutrient enrichment of hemolymph (blood) to perturb this regulatory module. We document factorspecific changes in fat body ilp1 and ilp2 mRNA, the bee's ILP-encoding genes, and confirm that our protocol affects social behavior. We show that ilp1 and ilp2 are regulated independently and differently and diverge in their specific expressionlocalization between fat body oenocyte and trophocyte cells. Insect ilp functions may be better understood by broadening research to account for expression in fat body and not only brain.

AB - Nutrient sensitive insulin-like peptides (ILPs) have profound effects on invertebrate metabolism, nutrient storage, fertility and aging. Many insects transcribe ILPs in specialized neurosecretory cells at changing levels correlated with life history. However, the major site of insect metabolism and nutrient storage is not the brain, but rather the fat body, where functions of ILP expression are rarely studied and poorly understood. Fat body is analogous to mammalian liver and adipose tissue, with nutrient stores that often correlate with behavior. We used the honey bee (Apis mellifera), an insect with complex behavior, to test whether ILP genes in fat body respond to experimentally induced changes of behavioral physiology. Honey bee fat body influences endocrine state and behavior by secreting the yolk protein precursor vitellogenin (Vg), which suppresses lipophilic juvenile hormone and social foraging behavior. In a two-factorial experiment, we used RNA interference (RNAi)-mediated vg gene knockdown and amino acid nutrient enrichment of hemolymph (blood) to perturb this regulatory module. We document factorspecific changes in fat body ilp1 and ilp2 mRNA, the bee's ILP-encoding genes, and confirm that our protocol affects social behavior. We show that ilp1 and ilp2 are regulated independently and differently and diverge in their specific expressionlocalization between fat body oenocyte and trophocyte cells. Insect ilp functions may be better understood by broadening research to account for expression in fat body and not only brain.

KW - Division of labor

KW - Nutrition

KW - Peripheral insulin-like signaling

KW - RNA-interference

KW - Vitellogenin

UR - http://www.scopus.com/inward/record.url?scp=79955045942&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79955045942&partnerID=8YFLogxK

U2 - 10.1242/jeb.050393

DO - 10.1242/jeb.050393

M3 - Article

C2 - 21490257

AN - SCOPUS:79955045942

VL - 214

SP - 1488

EP - 1497

JO - Journal of Experimental Biology

JF - Journal of Experimental Biology

SN - 0022-0949

IS - 9

ER -